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* Injection of intradermal antigen into the skin.
 
* Injection of intradermal antigen into the skin.
 
* A skin reaction (infiltration of lymphocytes and monocytes) peaking at 48-72 hours indicates prior exposure to the antigen or ongoing infection.
 
* A skin reaction (infiltration of lymphocytes and monocytes) peaking at 48-72 hours indicates prior exposure to the antigen or ongoing infection.
* It is used for TB testing cattle; the antigen is purified protein derivative-PPD from ''Mycobacterium tuberculosis''.
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* It is used for TB testing cattle; the antigen is purified protein derivative-PPD from [[Mycobacteria spp.|''Mycobacterium tuberculosis'']].
 
* Presently in the UK the animal is culled if it has a positive skin reaction.
 
* Presently in the UK the animal is culled if it has a positive skin reaction.
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