Intracytoplasmic Sperm Injection - Anatomy & Physiology

• Common in human, effective for laboratory mice.
• Sperm need not be capacitated, they can be epididymal,testicular or even a spermatid.
  • Only requirement is genes and cytoplasm
  • Even dead freeze-dried sperm have been effective in mice.
• Abnormalities are frequent.
• Power is in the hands of the operator, as it bypasses the process of selection in the female tract.
  • Operator must select a single sperm to inject directly into the Oocyte under a microscope.
• Oocyte then placed in cell culture for development.