Difference between revisions of "Intracytoplasmic Sperm Injection - Anatomy & Physiology"

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m (Text replace - "[[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology" to "[[Sperm in the Female Tract - Anatomy & Physiology")
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* Common in human, effective for laboratory mice.
 
* Common in human, effective for laboratory mice.
* Sperm need not be [[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology#Capacitation|capacitated]], they can be epididymal,testicular or even a spermatid.
+
* Sperm need not be [[Sperm in the Female Tract - Anatomy & Physiology#Capacitation|capacitated]], they can be epididymal,testicular or even a spermatid.
 
** Only requirement is genes and cytoplasm
 
** Only requirement is genes and cytoplasm
 
** Even dead freeze-dried sperm have been effective in mice.
 
** Even dead freeze-dried sperm have been effective in mice.
 
* Abnormalities are frequent.
 
* Abnormalities are frequent.
* Power is in the hands of the operator, as it bypasses the process of [[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology|selection in the female tract]].
+
* Power is in the hands of the operator, as it bypasses the process of [[Sperm in the Female Tract - Anatomy & Physiology|selection in the female tract]].
 
** Operator must select a single sperm to inject directly into the [[Oogenesis - Anatomy & Physiology|Oocyte]] under a microscope.
 
** Operator must select a single sperm to inject directly into the [[Oogenesis - Anatomy & Physiology|Oocyte]] under a microscope.
 
* [[Oogenesis - Anatomy & Physiology|Oocyte]] then placed in cell culture for development.
 
* [[Oogenesis - Anatomy & Physiology|Oocyte]] then placed in cell culture for development.

Revision as of 15:20, 8 December 2010

  • Common in human, effective for laboratory mice.
  • Sperm need not be capacitated, they can be epididymal,testicular or even a spermatid.
    • Only requirement is genes and cytoplasm
    • Even dead freeze-dried sperm have been effective in mice.
  • Abnormalities are frequent.
  • Power is in the hands of the operator, as it bypasses the process of selection in the female tract.
    • Operator must select a single sperm to inject directly into the Oocyte under a microscope.
  • Oocyte then placed in cell culture for development.