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Also known as: '''''CFT — Complement Fixation Test'''''
==Introduction==
Complement fixation is a test that exploits the fact that antibody-antigen complexes are able
Complement fixation is a test that exploits the fact that antibody-antigen complexes are able
to activate the complement system, using the mechanism to show the presence of a specific antibody in a serum sample.
to activate the complement system, using the mechanism to show the presence of a specific antibody in a serum sample.
==Method==
A mixture of antibody, complement and antigen is incubated and an indicator system (normally antibody-coated sheep red blood cells) is added.
A mixture of antibody, complement and antigen is incubated and an indicator system (normally antibody-coated sheep red blood cells) is added.
*As the complement reaction with the immune complex produces no visible result, sheep red blood cells are coated with anti-sheep red blood cell antibody. The complement in the mixture will react with this antibody and lyse the cells.
*As the complement reaction with the immune complex produces no visible result, sheep red blood cells are coated with anti-sheep red blood cell antibody. The complement in the mixture will react with this antibody and lyse the cells.
An indication of a positive test is the absence of lysis of the red blood cells, as the complement has already been used up by the antigen-antibody system. Controls are included to ensure that none of the reagents have taken up the complement non-specifically (e.g. contaminated serum)
An indication of a positive test is the absence of lysis of the red blood cells, as the complement has already been used up by the antigen-antibody system. Controls are included to ensure that none of the reagents have taken up the complement non-specifically (e.g. contaminated serum)
==Applications==
*'''Wasserman reaction'''- used in the diagnosis of syphilis, the test consists of a mixture of Wasserman antigen, dilutions of the patients serum and complement (normally sourced from guinea pigs).
*'''Wasserman reaction'''- used in the diagnosis of syphilis, the test consists of a mixture of Wasserman antigen, dilutions of the patients serum and complement (normally sourced from guinea pigs).
*Virus detection- tissue samples are innoculated with blood or tissue samples from a patient and tested using complement fixation
*Virus detection- tissue samples are innoculated with blood or tissue samples from a patient and tested using complement fixation
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[[Category:Immunological Testing]]
[[Category:Immunological Testing]]