Changes

==Pathology==  

==Pathology==  

'''Histological examination''' reveals a '''systemic infection of all organs''' and '''skeletal muscles''', with the most severe changes being '''diffuse interstitial necrosis''' of the anterior and posterior '''kidney and systemic haemorrhages'''. '''Focal necrosis in the liver'' and '''spleen''' are also generally seen as '''pale grey/white lesions'''.  Skeletal muscle and areas of necrosis within internal organ tissue can be '''infiltrated with macrophages''', that phagocytose the bacteria but do not destroy it <ref name="name">   (Miyazaki and Plumb, 1985</ref>; <ref name="name">   Klesius et al., 1991</ref>; <ref name="name">   Klesius, 1993;)</ref>.

'''Histological examination''' reveals a '''systemic infection of all organs''' and '''skeletal muscles''', with the most severe changes being '''diffuse interstitial necrosis''' of the anterior and posterior '''kidney and systemic haemorrhages'''. '''Focal necrosis in the liver'' and '''spleen''' are also generally seen as '''pale grey/white lesions'''.  Skeletal muscle and areas of necrosis within internal organ tissue can be '''infiltrated with macrophages''', that phagocytose the bacteria but do not destroy it <ref name="Miyazaki and Plumb, 1985">Miyazaki, T., Plumb, J,A., (1985)  '''Histopathology of Edwardsiella ictaluri in channel catfish, Ictalurus punctatus (Rafinesque)'''. ''Journal of Fish Diseases'', 8(4):389-392.(</ref>, <ref name="Klesius et al., 1991">Klesius, P., Johnson, K., Durborow, R., Vinitnantharat, S., (1991)  '''Development and evaluation of an enzyme-linked immunosorbent assay for catfish serum antibody to Edwardsiella ictaluri'''. ''Journal of Aquatic Animal Health'', 3(2):94-99.</ref>, <ref name="Klesius, 1993">Klesius, P.H., (1993)   '''Rapid enzyme-linked immunosorbent tests for detecting antibodies to Edwardsiella ictaluri in channel catfish, Ictalurus punctatus, using exoantigen'''. ''Veterinary Immunology and Immunopathology'', 36(4):359-368.</ref>.

==Diagnosis==

==Diagnosis==