Changes

'E. tarda' can be '''isolated on brain–heart infusion (BHI) agar''' or '''trypton soya agar (TSA)''' with inocula from infected internal organs or muscle and '''form small, round, convex transparent colonies''' (0.5 mm in diameter)after 24-48 hours.  On '''''Edwardsiella isolation media (EIM)''''', it forms '''small green colonies with black centres'''.   

'E. tarda' can be '''isolated on brain–heart infusion (BHI) agar''' or '''trypton soya agar (TSA)''' with inocula from infected internal organs or muscle and '''form small, round, convex transparent colonies''' (0.5 mm in diameter)after 24-48 hours.  On '''''Edwardsiella isolation media (EIM)''''', it forms '''small green colonies with black centres'''.   

'''Indirect FAT (detecting antibodies) and enzyme-linked immunosorbent assay (ELISA) test ''' is used to confirm the presence of ''E. tarda''. There is no serological cross-reactivity between E. tarda and E. Ictaluri. More recently, a '''loop-mediated isothermal amplification (LAMP)''' for rapid and sensitive detection of ''E. tarda'' has been developed(Savan et al. (2004)

'''Indirect FAT (detecting antibodies) and enzyme-linked immunosorbent assay (ELISA) test ''' is used to confirm the presence of ''E. tarda''. There is no serological cross-reactivity between E. tarda and E. Ictaluri. More recently, a '''loop-mediated isothermal amplification (LAMP)''' for rapid and sensitive detection of ''E. tarda'' has been developed <ref name="Savan et al, 2004">Savan, R., Igarashi, A., Matsuoka, S., Sakai, M., (2004) '''Sensitive and rapid detection of edwardsiellosis in fish by a loop-mediated isothermal amplification method'''. ''Applied and Environmental Microbiology'', 70(1):621-624.</ref>.

==Treatment==

==Treatment==