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The '''direct and indirect [[Immunofluorescence|fluorescent antibody tests]]''' are among the most '''effective, simple and rapid serological methods''' of identification for most Mycoplasma species.<ref> Rosendal, S., Black, F. T (1972) '''Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies'''. Acta Pathologica et Microbiologica Scandinavica, 80:615-622.</ref> Several forms have been described, the most commonly used one is the  indirect fluorescent antibody (IFA) test which is applied to unfixed colonies on agar.

The '''direct and indirect [[Immunofluorescence|fluorescent antibody tests]]''' are among the most '''effective, simple and rapid serological methods''' of identification for most Mycoplasma species.<ref> Rosendal, S., Black, F. T (1972) '''Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies'''. Acta Pathologica et Microbiologica Scandinavica, 80:615-622.</ref> Several forms have been described, the most commonly used one is the  indirect fluorescent antibody (IFA) test which is applied to unfixed colonies on agar.

The complement fixation test (CFT) and the indirect haemagglutination test (IHA) are serological methods of diagnosis, as is the [[ELISA testing|ELISA]]. These have varying degrees of efficacy.

The [[Complement Fixation|complement fixation test (CFT)]] and the indirect haemagglutination test (IHA) are serological methods of diagnosis, as is the [[ELISA testing|ELISA]]. These have varying degrees of efficacy.

Diagnostic systems based on '''PCR''' have been developed for the rapid detection, identification and differentiation of members of the ''M. mycoides'' cluster and the specific identification of ''M. capricolum'' subsp. ''capripneumoniae''<ref>Bashiruddin, J. B., Taylor, T. K., Gould, A. R (1994) '''A PCR-based test for the specific identification of Mycoplasma mycoides subspecies mycoides SC'''. Journal of Veterinary Diagnostic Investigation, 6(4):428-434; 14</ref> <ref>Hotzel, H., Sachse, K., Pfützner, H (1996) '''A PCR scheme for differentiation of organisms belonging to the Mycoplasma mycoides cluster'''. Veterinary Microbiology, 49(1/2):31-43; 21</ref>

Diagnostic systems based on '''PCR''' have been developed for the rapid detection, identification and differentiation of members of the ''M. mycoides'' cluster and the specific identification of ''M. capricolum'' subsp. ''capripneumoniae''<ref>Bashiruddin, J. B., Taylor, T. K., Gould, A. R (1994) '''A PCR-based test for the specific identification of Mycoplasma mycoides subspecies mycoides SC'''. Journal of Veterinary Diagnostic Investigation, 6(4):428-434; 14</ref> <ref>Hotzel, H., Sachse, K., Pfützner, H (1996) '''A PCR scheme for differentiation of organisms belonging to the Mycoplasma mycoides cluster'''. Veterinary Microbiology, 49(1/2):31-43; 21</ref>

|flashcards = [[Contagious Caprine Pleuropneumonia Flashcards]]

|flashcards = [[Contagious Caprine Pleuropneumonia Flashcards]]

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== References ==

== References ==

Wesonga, H. O., Litamoi, J. K., Kagumba, M., Wakhusama, E (1993) '''Relationship between clinical signs and early lesions of contagious caprine pleuropneumonia caused by Mycoplasma strain F38'''. ''Small Ruminant Research,'' 10(1):45-54; 15  

Wesonga, H. O., Litamoi, J. K., Kagumba, M., Wakhusama, E (1993) '''Relationship between clinical signs and early lesions of contagious caprine pleuropneumonia caused by Mycoplasma strain F38'''. ''Small Ruminant Research,'' 10(1):45-54; 15  

[[Category:To Do - CABI review]]

 

[[Category:CABI Expert Review]]

[[Category:Respiratory Diseases - Goat]]

[[Category:Respiratory Diseases - Goat]]

[[Category:Respiratory Bacterial Infections]]

[[Category:Respiratory Bacterial Infections]]