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Also known as: '''''EEV — Alphavirus — Eastern Equine Encephalitis Virus, EEE — Western Equine Encephalitis Virus, WEE — Venezuelan Equine Encephalitis Virus, VEE
 
Also known as: '''''EEV — Alphavirus — Eastern Equine Encephalitis Virus, EEE — Western Equine Encephalitis Virus, WEE — Venezuelan Equine Encephalitis Virus, VEE
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*Virus isolation is the most definitive diagnostic method for EEE or WEE.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Brain is preferred, but virus has also been isolated from the liver and spleen.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>. Samples of these tissues should be taken in duplicate, one set for virus isolation and the other placed in formalin for histopathology.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>. Viral isolation specimens should be sent frozen unless they can be received refrigerated within 48 hours of sampling.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Unless clinical signs persist for more than 5days prior to death, EEE virus is frequently isolated from equine brain tissue.  WEE virus, however, is rarely isolated from tissues of infected horses.  Newborn mice, chicken embryos and a number of cell culture systems can be used for virus isolation.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Virus may also be isolated from cerebrospinal fluid (CSF) of acutely infected horses.<ref name="duplicate">Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010</ref>
 
*Virus isolation is the most definitive diagnostic method for EEE or WEE.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Brain is preferred, but virus has also been isolated from the liver and spleen.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>. Samples of these tissues should be taken in duplicate, one set for virus isolation and the other placed in formalin for histopathology.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>. Viral isolation specimens should be sent frozen unless they can be received refrigerated within 48 hours of sampling.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Unless clinical signs persist for more than 5days prior to death, EEE virus is frequently isolated from equine brain tissue.  WEE virus, however, is rarely isolated from tissues of infected horses.  Newborn mice, chicken embryos and a number of cell culture systems can be used for virus isolation.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Virus may also be isolated from cerebrospinal fluid (CSF) of acutely infected horses.<ref name="duplicate">Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010</ref>
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==Literature Search==
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{{Learning
[[File:CABI logo.jpg|left|90px]]
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|literature search = [http://www.cabdirect.org/search.html?q=title%3A%28%22Equine+Encephalitis+Virus%22%29+AND+od%3A%28horses%29 Equine encephalitis virus in horses]
 
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Use these links to find recent scientific publications via CAB Abstracts (log in required unless accessing from a subscribing organisation).
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[http://www.cabdirect.org/search.html?q=title%3A%28%22Equine+Encephalitis+Virus%22%29+AND+od%3A%28horses%29 Equine encephalitis virus in horses]
      
[http://www.cabdirect.org/search.html?rowId=1&options1=AND&q1=%22Equine+Encephalitis+Virus%22&occuring1=title&rowId=2&options2=NOT&q2=horses&occuring2=od&rowId=3&options3=AND&q3=&occuring3=freetext&x=42&y=9&publishedstart=yyyy&publishedend=yyyy&calendarInput=yyyy-mm-dd&la=any&it=any&show=all Equine encephalitis virus in other species, including laboratory animals]
 
[http://www.cabdirect.org/search.html?rowId=1&options1=AND&q1=%22Equine+Encephalitis+Virus%22&occuring1=title&rowId=2&options2=NOT&q2=horses&occuring2=od&rowId=3&options3=AND&q3=&occuring3=freetext&x=42&y=9&publishedstart=yyyy&publishedend=yyyy&calendarInput=yyyy-mm-dd&la=any&it=any&show=all Equine encephalitis virus in other species, including laboratory animals]
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==References==
 
==References==
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[[Category:Togaviridae]]
 
[[Category:Togaviridae]]
Author, Donkey, Bureaucrats, Administrators
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