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* Normally 80ml is delivered through the cervix to reach the [[Female_Reproductive_Tract_-The_Uterus_-_Anatomy_%26_Physiology|uterus]] by gravitational flow.
 
* Normally 80ml is delivered through the cervix to reach the [[Female_Reproductive_Tract_-The_Uterus_-_Anatomy_%26_Physiology|uterus]] by gravitational flow.
 
* Uses 2-3 billion sperm per insemination (60 billion in a normal ejaculate), so is relatively inefficient only giving ~20 inseminations per ejaculate.
 
* Uses 2-3 billion sperm per insemination (60 billion in a normal ejaculate), so is relatively inefficient only giving ~20 inseminations per ejaculate.
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'''MARE'''
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* Gloved, lubricated hand is inserted directly into the vagina.
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* Index finger is used to guide the insemination pipette into the cervical lumen.
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* Marker used to guage the depth of insemination.
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'''BITCH'''
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* Vulva is elevated manually so that the ventral 'tilt' of the vestibule is removed.
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* Allows the insemination pipette to be inserted with relative ease.
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* Hindquarters of the bitch should be elevated for ~5 minutes after deposition of the semen to allow pooling in the cranial vagina and caudal cervix.
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