Changes

* Direct follicle aspiration:  

* Direct [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] aspiration:  

** A hyperdermic needle is inserted into the follicle.

** A hyperdermic needle is inserted into the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]].

** Follicular fluid is aspirated.

** Follicular fluid is aspirated.

** Follicular fluid forcefully returned to the follicle.

** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]].

** Repeated 2-3 times to dislodge oocytes.

** Repeated 2-3 times to dislodge oocytes.

** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_-_Anatomy_%26_Physiology|rectum]].

** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_-_Anatomy_%26_Physiology|rectum]].

** Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.

** Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.

** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.

** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized.

** Hypodermic needle is advanced through the vaginal wall into the antral follicle.

** Hypodermic needle is advanced through the vaginal wall into the antral follicle.

** Follicular fluid containing the oocyte is aspirated under constant vacuum.

** Follicular fluid containing the oocyte is aspirated under constant vacuum.