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Semen Sexing - Anatomy & Physiology (view source)
Revision as of 10:14, 17 July 2008
, 10:14, 17 July 2008no edit summary
<big><center>[[Reproductive_System#Reproductive_Technologies|'''BACK TO REPRODUCTIVE TECHNOLOGIES''']]</center></big>
<big><center>[[Reproductive_System#Reproductive_Technologies|'''BACK TO REPRODUCTIVE TECHNOLOGIES''']]</center></big>
== Principle ==
* X and Y chromosomes contain different quantities of DNA.
* X-bearing sperm contain 2.8-4.2% more DNA (species dependent) than Y-bearing sperm.
* This allows them to be sorted into two subpopulations.
* Separation requires the uptake of DNA stain/dye called Fluorochrome into living and dead sperm.
* X-bearing sperm take up more dye than Y-bearing sperm.
* Vital dyes emit light at a specific wavelength when excited or activated.
** X-bearing sperm will emit a larger signal than Y-bearing sperm.
* This allows the sperm to be sorted by '''flow cytometry'''.
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== Flow Cytometry ==