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Blood Sample Collection (view source)
Revision as of 17:29, 21 February 2022
, 17:29, 21 February 2022no edit summary
Always label your sample tubes with permanent marker immediately with the animal's details and the date and time of collection. Serum samples can be processed by allowing the blood to clot in an upright position and removing the serum from the tube prior to sending the sample for analysis to avoid haemolysis (bursting of the red blood cells into the serum) during transit. A more ideal method of serum separation is to centrifuge the sample -balance the centrifuge by adding a tube filled with the same volume of fluid in the opposite well in the centrifuge for safety.
Always label your sample tubes with permanent marker immediately with the animal's details and the date and time of collection. Serum samples can be processed by allowing the blood to clot in an upright position and removing the serum from the tube prior to sending the sample for analysis to avoid haemolysis (bursting of the red blood cells into the serum) during transit. A more ideal method of serum separation is to centrifuge the sample -balance the centrifuge by adding a tube filled with the same volume of fluid in the opposite well in the centrifuge for safety.
=== Sampling ===
Anticoagulated blood is required for cell counts. If there are any visible clots, even small ones, the sample is discarded, as the WBC and platelet counts will be inaccurate. EDTA is the best anticoagulant to preserve cell detail but this does not provide such good cell morphology as a well prepared fresh smear. Heparin can be used but produces poor staining of cells. EDTA tubes should be completely filled to the level indicated. An excess of EDTA causes red cell shrinkage and decreased PCV. Overfilling may lead to clotting. Blood should be analysed within 2-3 hours or kept refrigerated. After 6-24 hours storage, RBCs swell leading to increased PCV and MCV with reduced mean corpuscular haemoglobin concentration (MCHC). Other red cell indices are not affected. Artefactual changes may result from excess suction on the syringe, over zealous agitation of the EDTA tube and high environmental temperatures, which can all cause haemolysis.
Sample ageing also increasesthe likelihood of haemolysis. Samples submitted to the laboratory should arrive within 2 days. Delays often resultin cell deterioration and lysis, therefore submission of a fresh smear is advisable.
References: Nation Wide Laboratories
=== Sample Type Required table ===
The following table is a guide to the sample type required for some specific laboratory tests - seek guidance from your processing laboratory for confirmation of sample requirements, which can vary depending on the equipment used to analyse the sample.
The following table is a guide to the sample type required for some specific laboratory tests - seek guidance from your processing laboratory for confirmation of sample requirements, which can vary depending on the equipment used to analyse the sample.
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