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Preparation and Examination of Blood Smear (view source)
Revision as of 16:06, 24 February 2022
, 16:06, 24 February 2022no edit summary
If parasitaemia is a concern, smears should be made from capillary blood (ear prick) since the concentration of parasites is higher in peripheral blood.
If parasitaemia is a concern, smears should be made from capillary blood (ear prick) since the concentration of parasites is higher in peripheral blood.
Well prepared blood smears should have an area where the blood cells are in a monolayer. Place a small drop of blood on the slide towards one end. Draw the spreader slide back to contact the drop and then move it forwards before the blood has spread along the width of the spreader slide. This will prevent the smear extending to the extremities of the slide, when cells can be lost over the edges. The smear should terminate before the end of the slide (Fig 1).
Well prepared blood smears should have an area where the blood cells are in a monolayer. Place a small drop of blood on the slide towards one end. Draw the spreader slide back to contact the drop and then move it forwards before the blood has spread along the width of the spreader slide. This will prevent the smear extending to the extremities of the slide, when cells can be lost over the edges. The smear should terminate before the end of the slide (Fig 1).
The area to be examined is the monolayer where 50% or less of cells are in contact. The differential count is performed, and cell morphology is assessed in the monolayer (Fig 2).
The area to be examined is the monolayer where 50% or less of cells are in contact. The differential count is performed, and cell morphology is assessed in the monolayer (Fig 2).
[[File:Nation Wide Laboratories.png|thumb|left|NationWide Laboratories]][[File:NationWide Laboratories Fig2.png|right|thumb|NationWide Laboratories ]]
== The Complete Blood Count ==
== The Complete Blood Count ==