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ELISA testing
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Revision as of 10:39, 22 August 2008
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10:39, 22 August 2008
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**Therefore they are easier to control and yield accurate results
**Therefore they are easier to control and yield accurate results
**However they are easily influenced by cross reactions and non-specific binding
**However they are easily influenced by cross reactions and non-specific binding
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'''Solid VS Fluid-phase'''
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An important consideration is the medium in which the assay is carried out, largely dependent on what is being tested:
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*Fluid-phase (''in solution'')
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**Main advantage is that the behaviour of molecules in solution is easier to predict
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*Solid-phase (surface of protein-binding material, e.g. plastic)
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**Easier to perform and more sensitive
===Non-competitive ELISA===
===Non-competitive ELISA===
Eayton
178
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