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==Introduction==

==Introduction==

[[Image:763px-Cryptosporidium parvum 01.jpg|thumb|right|150px|Immunofluorescence of ''Cryptosporidium parvum'' spores]]

[[Image:763px-Cryptosporidium parvum 01.jpg|thumb|right|150px|Immunofluorescence of ''Cryptosporidium parvum'' spores]]

Immunofluorescence is a technique used to detect cell or tissue-associated antigens using antibodies labelled with fluorescent tags. The stained tissues are then detected by immunofluorescence microscopy (qualitative) or flow cytometry (quantitative). Antibodies bind stably and specifically to their corresponding antigen and the technique makes use of the fact that they can be coupled to fluorescent dyes, such as fluorescein and rhodamine, with no effect on specificity. These conjugates bind to antigens present in a sample and can then be visualised under a microscope with a suitable light source, such as UV light. Conversely, the technique can also be used to detect antibodies directed against antigens known to exist in a sample.  

Immunofluorescence is a technique used to detect cell or tissue-associated antigens using antibodies labelled with fluorescent tags. The stained tissues are then detected by immunofluorescence microscopy (qualitative) or flow cytometry (quantitative). Antibodies bind stably and specifically to their corresponding antigen and the technique makes use of the fact that they can be coupled to fluorescent dyes, such as fluorescein and rhodamine, with no effect on specificity. These conjugates bind to antigens present in a sample and can then be visualised under a microscope with a suitable light source, such as UV light.

==Fluorescent dyes==

==Fluorescent dyes==