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→Commonly used fluorochromes
===Commonly used fluorochromes===
===Commonly used fluorochromes===
*'''Fluorescein:''' organic (carbon-based) dye, most widely used. Absorbs blue (490nm) and emits yellow green fluorescence(517nm)
*'''Fluorescein:''' organic (carbon-based) dye, most widely used. Absorbs blue (490nm) and emits yellow green fluorescence (517nm)
*'''Rhodamine:''' organic dye, absorbs yellow-green light and emits deep red fluorescence (546nm).
*'''Rhodamine:''' organic dye, absorbs yellow-green light and emits deep red fluorescence (546nm).
**As fluorescein and rhodamine fluorescences are easy to distinguish from one another, it is possible to conjugate them to different antibodies and simultaneously visualise two different antigens on the same cell or tissue.
**As fluorescein and rhodamine fluorescences are easy to distinguish from one another, it is possible to conjugate them to different antibodies and simultaneously visualise two different antigens on the same cell or tissue.