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* The pre-pubertal female has a lack of gonadal oestradiol to stimulate the surge centre.
 
* The pre-pubertal female has a lack of gonadal oestradiol to stimulate the surge centre.
* The tonic GnRH centre stimulates LH pulses from the Anterior Pituitary Gland. The amplitude of these pulses is the same as in the post-pubertal female, but the frequency is much lower.
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* The tonic GnRH centre stimulates LH pulses from the [[Pituitary_Gland_-_Anatomy_%26_Physiology#Anterior_Pituitary_Gland|Anterior Pituitary Gland]]. The amplitude of these pulses is the same as in the post-pubertal female, but the frequency is much lower.
* Prior to puberty, low frequency GnRH pulses do not provide enough stimulus for the Anterior Pituitary Gland to release FSH and LH at high levels.
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* Prior to puberty, low frequency GnRH pulses do not provide enough stimulus for the [[Pituitary_Gland_-_Anatomy_%26_Physiology#Anterior_Pituitary_Gland|Anterior Pituitary Gland]] to release FSH and LH at high levels.
 
* Therefore, follicular development does not result in high levels of circulating oestradiol.
 
* Therefore, follicular development does not result in high levels of circulating oestradiol.
 
* Oestradiol remains below the threshold that is needed to trigger the GnRH surge centre.
 
* Oestradiol remains below the threshold that is needed to trigger the GnRH surge centre.
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