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**Separation of the oocyte from the granulosal cells by a thick layer of material known as the zona pellucida.
 
**Separation of the oocyte from the granulosal cells by a thick layer of material known as the zona pellucida.
 
* This development is driven by Gdf9 and BMP15. Knockouts of these genes result in the failure of primordial follicles to develop into primary follicles.  These are normally found within the oocyte itself.
 
* This development is driven by Gdf9 and BMP15. Knockouts of these genes result in the failure of primordial follicles to develop into primary follicles.  These are normally found within the oocyte itself.
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