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60 bytes added ,  10:03, 11 September 2008
Line 64: Line 64:  
# Enzyme-labeled specific antibody is added- attaches to bound antigen
 
# Enzyme-labeled specific antibody is added- attaches to bound antigen
 
# Wash
 
# Wash
# Enzyme substrate is added
+
# Enzyme substrate is added (with dye for visualisation)
 
''visualised product = amount of antigen in the serum''
 
''visualised product = amount of antigen in the serum''
   Line 80: Line 80:     
'''Indirect Method''' (for antibody detection)
 
'''Indirect Method''' (for antibody detection)
# Antigen is adsorbed onto solid phase
+
# Specific antigen (i.e. not from sample) is adsorbed onto solid phase
 
# Wash
 
# Wash
 
# Serum is added- any specific antibody present in sample binds to antigen
 
# Serum is added- any specific antibody present in sample binds to antigen
Line 96: Line 96:  
# Wash
 
# Wash
 
# Enzyme substrate is added
 
# Enzyme substrate is added
''visualised product = amount of enzyme-labelled antibody''
+
''visualised product = amount of antigen in serum sample''
    
'''Direct Antigen Competition''' (for antigen detection)
 
'''Direct Antigen Competition''' (for antigen detection)
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