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ELISA testing
(view source)
Revision as of 10:03, 11 September 2008
60 bytes added
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10:03, 11 September 2008
→Techniques
Line 64:
Line 64:
# Enzyme-labeled specific antibody is added- attaches to bound antigen
# Enzyme-labeled specific antibody is added- attaches to bound antigen
# Wash
# Wash
−
# Enzyme substrate is added
+
# Enzyme substrate is added
(with dye for visualisation)
''visualised product = amount of antigen in the serum''
''visualised product = amount of antigen in the serum''
Line 80:
Line 80:
'''Indirect Method''' (for antibody detection)
'''Indirect Method''' (for antibody detection)
−
#
Antigen
is adsorbed onto solid phase
+
#
Specific antigen (i.e. not from sample)
is adsorbed onto solid phase
# Wash
# Wash
# Serum is added- any specific antibody present in sample binds to antigen
# Serum is added- any specific antibody present in sample binds to antigen
Line 96:
Line 96:
# Wash
# Wash
# Enzyme substrate is added
# Enzyme substrate is added
−
''visualised product = amount of
enzyme-labelled antibody
''
+
''visualised product = amount of
antigen in serum sample
''
'''Direct Antigen Competition''' (for antigen detection)
'''Direct Antigen Competition''' (for antigen detection)
Eayton
178
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