* To reduce cost and improve pregnancy rates, the following regime is used:
* To reduce cost and improve pregnancy rates, the following regime is used:
** All animals are injected with PGF2α on the same day and observed for oestrus during the following 5 days.
** All animals are injected with PGF2α on the same day and observed for oestrus during the following 5 days.
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* The length of time between the injection and onset of oestrus does vary slightly, depending on the ovarian status at the time of the injection. If there is a large, dominant follicle, the time until oestrus onset will be short, whereas if only small follicles are present, the period will be longer.
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* Lactating dairy cows have a more variable interval between the injection and onset of oestrus.
** Any identified in oestrus are inseminated
** Any identified in oestrus are inseminated
** Any not identified as being in oestrus receive a second injection of prostaglandin followed by artificial insemination.
** Any not identified as being in oestrus receive a second injection of prostaglandin followed by artificial insemination.