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Rhabdoviridae
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Revision as of 21:45, 13 October 2008
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21:45, 13 October 2008
→Diagnosis
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=Diagnosis=
=Diagnosis=
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*'''Isolate''' and monitor for clinical signs
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*In the field:
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**Remove brain core sample via straw through occipital foramen in the direction of an eye
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**Place contents in a 50% glyerol/PBS for FAT or virus isolation, or in 10% formaldehyde for histology
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**Whole head can also be removed and sent to lab
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*In the lab:
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**'''Fluorescent Antibody Test (FAT)''' on smears or frozen sections of hippocampus or cerebellum should show presence of Negri bodies
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***Being replaced by testing for monoclonal antibodies to nucleoprotein
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**Histological detection of Negri bodies using Mann's or silver stains
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**Intracerebral inoculation of mice followed by FAT testing of brain smears of affected mice
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**RT-PCR on brain or saliva can be performed to determine genotype
=Rabies Control=
=Rabies Control=
*Antigenicity allows a single antigenic type to vaccinate
*Antigenicity allows a single antigenic type to vaccinate
Bepocock
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