Changes

*Anaerobic culture with hydrogen supplement and 5-10% carbon dioxide for 48 hours

*Anaerobic culture with hydrogen supplement and 5-10% carbon dioxide for 48 hours

*Colonies of ''C. perfringens'' are 5mm diameter, circular, flat and grey and surrounded by a zone of double haemolysis

*Colonies of ''C. perfringens'' are 5mm diameter, circular, flat and grey and surrounded by a zone of double haemolysis

*Positive cAMP test with ''Sreptococci agalactiae''

*Positive cAMP test with ''Streptococci agalactiae''

*Biochemical tests

*Biochemical tests

*Toxins identified in body fluids by toxin neutralisation or protection tests in lab animals

*Toxins identified in body fluids by toxin neutralisation or protection tests in lab animals

*Post mortem  

*Post mortem  

*Gram positive rods present on intestinal smears suggests clostridial enterotoxaemia

*Gram positive rods present on intestinal smears suggests clostridial enterotoxaemia

===Neurotoxic clostridia===

===Neurotoxic clostridia===