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Also known as immunoblotting, the Western blot technique is used to identify specific proteins or antibodies in complex mixtures and has largely overtaken the use immunoelectrophoresis in research and diagnosis.

Also known as immunoblotting, the Western blot technique is used to identify specific proteins or antibodies in complex mixtures and has largely overtaken the use of immunoelectrophoresis in research and diagnosis.

===Principle===

===Principle===

===Method===

===Method===

# Protein mixture is treated with sodium dodecyl sulfate (SDS), a strong dissociating agent

# Protein mixture is treated with sodium dodecyl sulfate (SDS), a strong dissociating agent

# Mixture is separated by electrophoresis- takes place in SDS polyacrylamide gel (SDS-PAGE)

# Mixture is separated by electrophoresis - takes place in SDS polyacrylamide gel (SDS-PAGE)

# The gel is removed and applied to a protein-binding sheet of nitrocellulose/nylon and an electric current passed through it

# The gel is removed and applied to a protein-binding sheet of nitrocellulose/nylon and an electric current passed through it

# The antigens of interest are detected using enzyme-linked antibodies

# The antigens of interest are detected using enzyme-linked antibodies