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Created page with 'Erythrocytes contain no nucleus and are thus only produced from stem cells. During the fetal stage production is in both the [[Liver - Anatomy & Physiology|liver…'
[[Erythrocytes|Erythrocytes]] contain no nucleus and are thus only produced from stem cells. During the fetal stage production is in both the [[Liver - Anatomy & Physiology|liver]] and [[Spleen - Anatomy & Physiology|spleen]] however production is transferred to the [[Bone Marrow - Anatomy & Physiology|bone marrow]] ([[Bone Marrow - Anatomy & Physiology#Red marrow|red marrow]]) in the final stages of gestation. Initially erythropoiesis occurs in all bones, however after puberty production is limited to membranous bones (ribs, vertebrae, pelvic bones etc.) as in the long bones adipose tissue replaces red marrow.
Erythrocyte stem cells contain no haemoglobin and it is only after a few cell divisions that pro-erythrocyte haemoglobin starts to enter the cells. When the haemoglobin levels are at the correct concentration the nucleus reduces in size and is removed from cell. Cells at this stage still have ribosomes and other organelles and stain differently to mature erythrocytes. They are called reticulocytes. Reticulocytes contain some RNA and continue to produce haemoglobin. After a few days these mature having reached a final haemoglobin concentration of 34%.
Reticulocytes and mature [[Erythrocytes|erythrocytes]] leave the [[Bone Marrow - Anatomy & Physiology|bone marrow]] by ‘squeezing’ through the capillary endothelial cells. Precursor to these stages cannot change shape and therefore remain in the [[Bone Marrow - Anatomy & Physiology|bone marrow]].
==Development==
[[Erythrocytes|Erythrocyte]] development is divided into several stages and at each stage the cell will divide several times. As [[Bone Marrow - Anatomy & Physiology|bone marrow]] does not act as storage, all erythrocytes produced are immediately released into the circulation.
[[Image:LH Developing Erythrocyte Histology.jpg|right|thumb|100px|'''Developing erythrocytes''' ©RVC 2008]]
===Stem cell ===
[[Erythrocytes|Erythrocytes]] develop from stem cells that give rise to precursors which eventually mature to form the erythrocyte. The first erythrocyte precursor, the proerythroblast, is derived from the Erythroid CFU ([[Overview of Haematopoiesis - WikiBlood#Colony Forming Units|CFU-E]]) which has differentiated from the multipotential myeloid stem cell ([[Overview of Haematopoiesis - WikiBlood#Colony Forming Units|CFU-GEMM]]). This process is controlled and influenced by a number of different factors including erythropoietin, IL-3, IL-4 and granulocyte-macrophage colony stimulating factor (GM-CSF).
===Nomenclature===
The naming of the stages of the erythrocyte precursors varies.
The main headings for this section refer to each stage in terms of a histological description. Proerythroblast, basophilic erythroblast, polychromatic erythroblast, orthochromatic erythroblast and polychromatophilic erythrocyte.
Another classification of the stages is based upon the [[Overview of Haematopoiesis - WikiBlood#Colony Forming Units|CFU-E]] being referred to as ‘rubri’. This system gives all developing blood cell lineages similar names. For example the first stage for erythrocytes is referred to as a [rubri]blast and the first stage for lymphocytes is called a [lympho]blast. The stages for the erythrocyte are rubriblast, prorubriblast, rubricyte and metarubricye.
Finally the stages can also be named according to the development of the normoblast stage. This gives the stages pronormoblast, early normoblast, intermediate normoblast, late normoblast, polychromatic cell.
===Stages===
[[Image:Erythropoiesis pathway.jpg|right|thumb|100px|'''Erythropoiesis pathway''']]
====Proerythroblast====
The first [[Erythrocytes|erythrocyte]] precursor produced directly from the [[Overview of Haematopoiesis - WikiBlood#Colony Forming Units|CFU-GEMM]] under the influence of erythropoietin. It has a large nucleus with free ribosomes in the cytoplasm giving the cytoplasm a basophilic appearance.
Alternative nomenclature: Pronormoblast or Rubriblast
====Basophilic erythroblast====
Smaller than the proerythroblast with a smaller nucleus but a more basophilic cytoplasm due to increased numbers of ribosomes in the cytoplasm. These ribosomes are involved in the production of haemoglobin.
Alternative nomenclature: Early normoblast or Rubriblast
====Polychromatic erythroblast====
This is the last precursor cell capable of mitosis and is smaller than the basophilic erythroblast. Its cytoplasm appears greyer due to the increased acidophilic staining caused by the presence of haemoglobin.
Alternative nomenclature: Intermediate normoblast or Prorubricyte
==== Orthochromatic erythroblast ====
Also called a normoblast. It is incapable of cell division and is only slightly larger than a mature erythrocyte but it does contain a small dense nucleus.
Alternative nomenclature: Late normoblast or Rubricyte
==== Polychromatophilic erythrocyte ====
Also called a reticulocyte and is formed when the nucleus is extruded from the normoblast. It still contains some ribosomes and therefore retains a slight basophilic stain. The clustering of the ribosomes forms a reticular network giving the name, reticulocyte. These cells can carry oxygen and enter the blood stream and are found in low concentrations in normal blood.
Alternative nomenclature: Polychromatic cell or Metarubricyte
====Erythrocyte====
Commonly called the red blood cell. It is the final product of erythropoiesis and is released from the [[Bone Marrow - Anatomy & Physiology|bone marrow]] into circulation.
Alternative nomenclature: None
==Nutritional factors==
Normal [[Erythrocytes|erythrocyte]] production requires protein, copper, iron, folic acid and vitamins B6 and B12. Deficiencies lead to inadequate production of haemoglobin.
===Iron===
Iron is found within each haem component of haemoglobin and binds oxygen.
===Folic acid and vitamin B12===
Required for DNA synthesis and thus important for the formation of new erythrocytes. Deficiency leads to the formation of macrocytes.
==Regulation==
Production of [[Erythrocytes|erythrocytes]] is regulated by '''erythropoietin''' (EPO) which is produced in the yolk sac, [[Liver - Anatomy & Physiology|liver]] and kidney from embryonic life until early neonatal life. In the adult it is produced in the kidneys. Erythropoietin is a glycoprotein hormone and acts under a negative feedback. Levels are normally kept low with sufficient amounts to maintain production of enough new erythrocytes. However, as the blood oxygen concentration decreases, the release of erythropoietin increases.
EPO is transported from kidneys to [[Bone Marrow - Anatomy & Physiology|bone marrow]] where it acts upon receptors on the CFU-E’s and causes differentiation into erythrocyte precursors. It also increases the rate of division and maturation of the developing erythrocyte precursors by increasing gene transcription. Thus it is not the number of [[Erythrocytes|erythrocytes]] but the oxygen concentration that regulates its release. EPO release can be affected by any form of renal pathology.
===Factors involved===
Erythropoietin production is directly and indirectly (via regulatory genes) increased by HIF-1 (Hypoxia inducible factor 1) which is a transcription activator that is oxygen sensitive.
Inflammatory induced release of interleukins reduces the secretion of erythropoietin.
==Links==
[[Lymphoreticular System - Anatomy & Physiology|Lymphoreticular System ]]
[[Immunology - WikiBlood|Immunology ]]
Erythrocyte stem cells contain no haemoglobin and it is only after a few cell divisions that pro-erythrocyte haemoglobin starts to enter the cells. When the haemoglobin levels are at the correct concentration the nucleus reduces in size and is removed from cell. Cells at this stage still have ribosomes and other organelles and stain differently to mature erythrocytes. They are called reticulocytes. Reticulocytes contain some RNA and continue to produce haemoglobin. After a few days these mature having reached a final haemoglobin concentration of 34%.
Reticulocytes and mature [[Erythrocytes|erythrocytes]] leave the [[Bone Marrow - Anatomy & Physiology|bone marrow]] by ‘squeezing’ through the capillary endothelial cells. Precursor to these stages cannot change shape and therefore remain in the [[Bone Marrow - Anatomy & Physiology|bone marrow]].
==Development==
[[Erythrocytes|Erythrocyte]] development is divided into several stages and at each stage the cell will divide several times. As [[Bone Marrow - Anatomy & Physiology|bone marrow]] does not act as storage, all erythrocytes produced are immediately released into the circulation.
[[Image:LH Developing Erythrocyte Histology.jpg|right|thumb|100px|'''Developing erythrocytes''' ©RVC 2008]]
===Stem cell ===
[[Erythrocytes|Erythrocytes]] develop from stem cells that give rise to precursors which eventually mature to form the erythrocyte. The first erythrocyte precursor, the proerythroblast, is derived from the Erythroid CFU ([[Overview of Haematopoiesis - WikiBlood#Colony Forming Units|CFU-E]]) which has differentiated from the multipotential myeloid stem cell ([[Overview of Haematopoiesis - WikiBlood#Colony Forming Units|CFU-GEMM]]). This process is controlled and influenced by a number of different factors including erythropoietin, IL-3, IL-4 and granulocyte-macrophage colony stimulating factor (GM-CSF).
===Nomenclature===
The naming of the stages of the erythrocyte precursors varies.
The main headings for this section refer to each stage in terms of a histological description. Proerythroblast, basophilic erythroblast, polychromatic erythroblast, orthochromatic erythroblast and polychromatophilic erythrocyte.
Another classification of the stages is based upon the [[Overview of Haematopoiesis - WikiBlood#Colony Forming Units|CFU-E]] being referred to as ‘rubri’. This system gives all developing blood cell lineages similar names. For example the first stage for erythrocytes is referred to as a [rubri]blast and the first stage for lymphocytes is called a [lympho]blast. The stages for the erythrocyte are rubriblast, prorubriblast, rubricyte and metarubricye.
Finally the stages can also be named according to the development of the normoblast stage. This gives the stages pronormoblast, early normoblast, intermediate normoblast, late normoblast, polychromatic cell.
===Stages===
[[Image:Erythropoiesis pathway.jpg|right|thumb|100px|'''Erythropoiesis pathway''']]
====Proerythroblast====
The first [[Erythrocytes|erythrocyte]] precursor produced directly from the [[Overview of Haematopoiesis - WikiBlood#Colony Forming Units|CFU-GEMM]] under the influence of erythropoietin. It has a large nucleus with free ribosomes in the cytoplasm giving the cytoplasm a basophilic appearance.
Alternative nomenclature: Pronormoblast or Rubriblast
====Basophilic erythroblast====
Smaller than the proerythroblast with a smaller nucleus but a more basophilic cytoplasm due to increased numbers of ribosomes in the cytoplasm. These ribosomes are involved in the production of haemoglobin.
Alternative nomenclature: Early normoblast or Rubriblast
====Polychromatic erythroblast====
This is the last precursor cell capable of mitosis and is smaller than the basophilic erythroblast. Its cytoplasm appears greyer due to the increased acidophilic staining caused by the presence of haemoglobin.
Alternative nomenclature: Intermediate normoblast or Prorubricyte
==== Orthochromatic erythroblast ====
Also called a normoblast. It is incapable of cell division and is only slightly larger than a mature erythrocyte but it does contain a small dense nucleus.
Alternative nomenclature: Late normoblast or Rubricyte
==== Polychromatophilic erythrocyte ====
Also called a reticulocyte and is formed when the nucleus is extruded from the normoblast. It still contains some ribosomes and therefore retains a slight basophilic stain. The clustering of the ribosomes forms a reticular network giving the name, reticulocyte. These cells can carry oxygen and enter the blood stream and are found in low concentrations in normal blood.
Alternative nomenclature: Polychromatic cell or Metarubricyte
====Erythrocyte====
Commonly called the red blood cell. It is the final product of erythropoiesis and is released from the [[Bone Marrow - Anatomy & Physiology|bone marrow]] into circulation.
Alternative nomenclature: None
==Nutritional factors==
Normal [[Erythrocytes|erythrocyte]] production requires protein, copper, iron, folic acid and vitamins B6 and B12. Deficiencies lead to inadequate production of haemoglobin.
===Iron===
Iron is found within each haem component of haemoglobin and binds oxygen.
===Folic acid and vitamin B12===
Required for DNA synthesis and thus important for the formation of new erythrocytes. Deficiency leads to the formation of macrocytes.
==Regulation==
Production of [[Erythrocytes|erythrocytes]] is regulated by '''erythropoietin''' (EPO) which is produced in the yolk sac, [[Liver - Anatomy & Physiology|liver]] and kidney from embryonic life until early neonatal life. In the adult it is produced in the kidneys. Erythropoietin is a glycoprotein hormone and acts under a negative feedback. Levels are normally kept low with sufficient amounts to maintain production of enough new erythrocytes. However, as the blood oxygen concentration decreases, the release of erythropoietin increases.
EPO is transported from kidneys to [[Bone Marrow - Anatomy & Physiology|bone marrow]] where it acts upon receptors on the CFU-E’s and causes differentiation into erythrocyte precursors. It also increases the rate of division and maturation of the developing erythrocyte precursors by increasing gene transcription. Thus it is not the number of [[Erythrocytes|erythrocytes]] but the oxygen concentration that regulates its release. EPO release can be affected by any form of renal pathology.
===Factors involved===
Erythropoietin production is directly and indirectly (via regulatory genes) increased by HIF-1 (Hypoxia inducible factor 1) which is a transcription activator that is oxygen sensitive.
Inflammatory induced release of interleukins reduces the secretion of erythropoietin.
==Links==
[[Lymphoreticular System - Anatomy & Physiology|Lymphoreticular System ]]
[[Immunology - WikiBlood|Immunology ]]