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Small quantities of conjugated bilirubin are found in the urine of normal dogs because it has a low renal threshold.
Small quantities of conjugated bilirubin are found in the urine of normal dogs because it has a low renal threshold.
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+The '''Van de Berg test''' can be used to distinguish conjugated from unconjugated bilirubin. Plasma from an icteric animal is treated with an aqueous solution of the reagent diazotised sulphanilic acid and this produces a red-purple colour reaction. The intensity of this colour is directly proportional to the amount of water soluble (conjugated ) bilirubin in the sample. Further addition of alcohol intensifies the colour if there is non-water soluble (unconjugated) bilirubin also present. The intensified colour is directly proportional to the total amount of bilirubin present in the sample and the difference between the two readings gives the amount of unconjugated bilirubin in the sample.
==Clinical Significance==
==Clinical Significance==