In most affected animals (pigs being an exception), large numbers of B. anthracis are found in the blood. A sample may be collected from an incision made in a well vascularised area such as the ear. In pigs, a small piece of lymphatic tissue should be collected aseptically and submitted. Diagnostic tests include bacterial culture, PCR and fluorescent antibody stains for demonstration of the bacillus in blood or tissue.
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In most affected animals (pigs being an exception), large numbers of B. anthracis are found in the blood. A sample from a fresh carcass may be collected from an incision made in a well vascularised area such as the ear. In pigs, a small piece of lymphatic tissue should be collected aseptically and submitted. Diagnostic tests include bacterial culture, PCR and fluorescent antibody stains for demonstration of the bacillus in blood or tissue.
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===Pathology===
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Bloody discharges from body orifices may be observed in carcasses. Rigor mortis is often absent or incomplete in dead animals. Failure of the blood to clot and splenomegaly are other important post mortem findings.