Often clinical signs are enough to make a presumptive diagnosis. Additionally urine can be collected and either left to settle or centrifued. This will confirm the presence of haemolysis rather than frank blood in the urine where the red cells will settle at the bottom.
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A definitive diagnosis can be made by isolating C.haemolyticum from the liver however it can be difficult to culture. Additionally the organism can be confirmed on fluorescent antibody or immunohistochemical testing of liver tissue.