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→Laboratory Tests
There is an array of laboratory tests available for the diagnosis of bluetongue, and the table below published by DEFRA<sup>1</sup> summarises these:
There is an array of laboratory tests available for the diagnosis of bluetongue, and the table below published by DEFRA<sup>1</sup> summarises these:
{| class="wikitable collapsible"
{| class="wikitable collapsible" width="75%"
| <center> <u> '''Test''' </u> </center>
| <center> <u> '''Test''' </u> </center>
| <center><u>'''Specimen Required'''</u> </center>
| <center><u>'''Specimen Required'''</u> </center>
|<center><u>'''Timescale'''</u> </center>
|<center><u>'''Timescale'''</u> </center>
|-
|-
|'''Virus Isolation'''
|<u>'''Virus Isolation'''</u>
|Whole EDTA blood
|Whole EDTA blood
|Virus
|Virus
|1 - 3 weeks
|1 - 3 weeks
|-
|-
|'''Antigen Detection''' <BR>Sandwich ELISA <BR>PCR <BR>Serum Neutralisation
|<U>'''Antigen Detection'''</U> <BR>Sandwich ELISA <BR>PCR <BR>Serum Neutralisation
|<BR>Whole heparin or EDTA blood; tissues <BR>Whole EDTA blood; tissues <BR>Whole heparin or EDTA blood; tissues
|<BR>Whole heparin or EDTA blood; tissues <BR>Whole EDTA blood; tissues <BR>Whole heparin or EDTA blood; tissues
|<BR>Antigen - group specific <BR>Viral RNA - group specific<BR>Serotype
|<BR>Antigen - group specific <BR>Viral RNA - group specific<BR>Serotype
|<BR>Blood: 5 - 14 days; Tissues: 4 hours <BR>2 days <BR>2 - 4 weeks
|<BR>Blood: 5 - 14 days; Tissues: 4 hours <BR>2 days <BR>2 - 4 weeks
|-
|-
|'''Antibody Detection''' <BR> Competition ELISA <BR> Serum Neutralisation <BR> Pathogenicity Testing in Sheep
|<U>'''Antibody Detection'''</U> <BR> Competition ELISA <BR> Serum Neutralisation <BR> Pathogenicity Testing in Sheep
|<BR>Serum <BR>Serum <BR> Virus isolate
|<BR>Serum <BR>Serum <BR> Virus isolate
|<BR>Antibody - group specific <BR>Antibody - serotype specific <BR>Virulence
|<BR>Antibody - group specific <BR>Antibody - serotype specific <BR>Virulence
|-
|-
|}
|}
===Pathology===
===Pathology===