Intradermal injections are made with 26-27 g needles. Sharp needles ensure less bruising and easier injections. Positive (histamine phosphate 1:100,000) and negative (0.9% buffered saline) control injections are made to help ensure test accuracy. Injections are made utilizing approximately 0.05-1.0 ml solution. Injections are made based on "bleb" size. The injection blebs should be of approximately the same size at each injection site. Reactions are most commonly evaluated subjectively on a scale of 0-4 (compared to the positive and negative controls). Reactions are read at approximately 15 minutes. False positive reactions may occur with irritants, or allergens used at too high a concentration. False negatives may occur as well with drug interference, host factors, time of year, and improper technique. | Intradermal injections are made with 26-27 g needles. Sharp needles ensure less bruising and easier injections. Positive (histamine phosphate 1:100,000) and negative (0.9% buffered saline) control injections are made to help ensure test accuracy. Injections are made utilizing approximately 0.05-1.0 ml solution. Injections are made based on "bleb" size. The injection blebs should be of approximately the same size at each injection site. Reactions are most commonly evaluated subjectively on a scale of 0-4 (compared to the positive and negative controls). Reactions are read at approximately 15 minutes. False positive reactions may occur with irritants, or allergens used at too high a concentration. False negatives may occur as well with drug interference, host factors, time of year, and improper technique. |