Changes

Routine haematology and biochemistry rarely show any abnormalities in dogs, but eosinophilia is often seen in cats¹. The measurement of total serum IgE levels is not useful in the diagnosis of atopic dermatitis as IgE levels do not significantly differ betweem atopic and normal animals^beale. IgE levels are also influence by the presence of parasites, vaccinations and breed, and so this test is not reliable

Routine haematology and biochemistry rarely show any abnormalities in dogs, but eosinophilia is often seen in cats¹. The measurement of total serum IgE levels is not useful in the diagnosis of atopic dermatitis as IgE levels do not significantly differ betweem atopic and normal animals^beale. IgE levels are also influence by the presence of parasites, vaccinations and breed, and so this test is not reliable

Allergen-specific IgE tests are only useful when a diagnosis of atopic dermatitis has already been reached by consideration of histoty and clinical exam, and by ruling out other causes of pruritus. The test is used to identify allergens for immunotherapy. the patient's serum is evaluated for IgE antibody directed against a panel of regional allergens (pollens, mold, dust, epidermals). Methodologies vary between laboratories, but in general, the principal is the same: the serum is allowed to react with an individual allergen extract . The unreacted antibodies are washed away, and then the allergen-bound antibody is detected using an IgE specific reagent (coupled to an enzyme or radioisotope). The IgE bound reagent is then quantified which should be proportional to the amount of allergen-specific IgE. Measurement depends on the assay utilized, but may use radiometric, fluorometric, or colorimetric measures.

Allergen-specific IgE tests are only useful when a diagnosis of atopic dermatitis has already been reached by consideration of histoty and clinical exam, and by ruling out other causes of pruritus. The test is used to identify allergens for immunotherapy by evaluating serum levels of IgE specific for a variety allergens. The exact technique varies between laboratories, but the principle is the same: Ser The unreacted antibodies are washed away, and then the allergen-bound antibody is detected using an IgE specific reagent (coupled to an enzyme or radioisotope). The IgE bound reagent is then quantified which should be proportional to the amount of allergen-specific IgE. Measurement depends on the assay utilized, but may use radiometric, fluorometric, or colorimetric measures.

 

Unfortunately, the laboratories are not subject to any standardization or quality control procedures. Reliability and repeatability of some of these commercial tests are unacceptable. Because there is no external regulation of these diagnostic laboratories, we must rely on the ethics of the companies performing the tests.

Besides laboratory inaccuracies, there are other factors which may influence test results: age, season during which the patient is tested, prior or current corticosteroid therapy.

Besides laboratory inaccuracies, there are other factors which may influence test results: age, season during which the patient is tested, prior or current corticosteroid therapy.