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===Laboratory Tests===
 
===Laboratory Tests===
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The diagnosis of subacute rumenal acidosis is aided by the measurement of the pH of rumenal fluid in a sample of animals. Cattle with the highest risk of SARA, i.e. those in early lactation, are selected and rumen fluid is obtained by rumenocentesis or, less commonly, stomach tube. Indicator paper or a pH meter is then used to measure the pH pf the samples. In general twelve animals should be sampled and if greater than 25-30% of the group have a pH less than 5.5, a diagnosis of SARA can be made. In a herd fed a total mixed ration as a single group, it might be useful to sample twelve recently  (14-21 days) calved cows and twelve animals with the maximal dry matter intake (60-80 days calved) in order to evaluate the adequacy of the diet for all animals and to identify at what stage the feeding is problematic. Samples should be taken 6-10 hours after access to fresh TMR, or 2-4 hours after grain feeding in component-fed herds.
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The diagnosis of subacute rumenal acidosis is aided by the measurement of the pH of rumenal fluid in a sample of animals. Cattle with the highest risk of SARA, i.e. those in early lactation, are selected and rumen fluid is obtained by rumenocentesis or, less commonly, stomach tube. Indicator paper or a pH meter is then used to measure the pH pf the samples. In general twelve animals should be sampled and if greater than 25-30% of the group have a pH less than 5.5, a diagnosis of SARA can be made. In a herd fed a total mixed ration as a single group, it might be useful to sample twelve recently  (14-21 days) calved cows and twelve animals with the maximal dry matter intake (60-80 days calved) in order to evaluate the adequacy of the diet for all animals and to identify at what stage the feeding is problematic. Samples should be taken 6-10 hours after access to fresh TMR, or 2-4 hours after grain feeding in component-fed herds. The amount of concentrate fed in component-fed herds can be varied before sampling to determine what level of feeding is associated with SARA.
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Milk fat depression is a poor and insensitive indicator of subacute ruminal acidosis in dairy herds. Cows and herds with severe subacute ruminal acidosis may have normal milk fat tests. Thus, it is vitally important not to exclude the diagnosis in a dairy herd that has a normal milk-fat test.
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Previously, measurement of milk fat depression has been advocated in the diagnosis of SARA. However, this is an insensitive indicator as some animals with severe SARA can have normal milk fat tests. It is therefore important not to exclude a diagnosis of SARA in an underpoerforming dairy herd that has a normal milk butterfat levels.
 
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Cows should be sampled four to
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eight hours after access to fresh TMR.
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Selection of cows in herds managed either on a
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hybrid TMR or a component-based ration is more problematic
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as the incidence of SARA is likely to be associated
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with parlour concentrate feeding and substitution
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effects. In such cases, it is advisable to sample cows
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receiving different levels of concentrate, ensuring that
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the amount of concentrate fed is recorded. Alternatively,
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two groups of cows may be sampled: first, those cows
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receiving maximal levels of concentrates and, secondly,
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those cows that may be overfed in t-he early postpartum
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period. Cows should be sampled two to three hours after
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receiving any parlour fed concentrates.
      
===Other Tests===
 
===Other Tests===
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