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Diagnosis of FIV is made by demonstrating the presence of antibodies against the virus. ELISA tests are available for in-house use, with some kits detecting antibody to the core protein p24 and others detect antibody to the envelope protein gp4l<sup>2</sup>. Non-haemolysed plasma or serum is used for performing the in-house ELISA. Results must be interpreted with caution.  
 
Diagnosis of FIV is made by demonstrating the presence of antibodies against the virus. ELISA tests are available for in-house use, with some kits detecting antibody to the core protein p24 and others detect antibody to the envelope protein gp4l<sup>2</sup>. Non-haemolysed plasma or serum is used for performing the in-house ELISA. Results must be interpreted with caution.  
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INTERPRETING A POSITIVE RESULT
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Once a cat acquires FIV infection, the antibodies created persist for life. This means that an ELISA test at any stage should give a positive rseult.
A positive test result is found in three situations:
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* PERSISTENTLY INFECTED CATS. FIV antibodies are
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associated with lifelong infection with this virus.
   
* KITTENS BORN TO AN FIV-POSITIVE QUEEN. Passive
 
* KITTENS BORN TO AN FIV-POSITIVE QUEEN. Passive
 
transfer of antibodies occurs via the milk, with the
 
transfer of antibodies occurs via the milk, with the
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These should be interpreted as equivocal results and the
 
These should be interpreted as equivocal results and the
 
cat should be retested using a different test.
 
cat should be retested using a different test.
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INTERPRETING A NEGATIVE RESULT
 
INTERPRETING A NEGATIVE RESULT
 
Negative results indicate one of three possibilities:
 
Negative results indicate one of three possibilities:
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