Changes

**RNA must be purified before converting to DNA for PCR

**RNA must be purified before converting to DNA for PCR

**False positives can occur easily by contamination with previously amplified DNA

**False positives can occur easily by contamination with previously amplified DNA

*May see animals that have discoloration of [[Oral Cavity - Tongue - Anatomy & Physiology|tongue]] due to having had FMD

*May see animals that have discoloration of [[Tongue - Anatomy & Physiology|tongue]] due to having had FMD

**In these cases take scraping of retropharyngeal region, put scrapings in transport medium and send for testing

**In these cases take scraping of retropharyngeal region, put scrapings in transport medium and send for testing

====Pathology====

====Pathology====

=====Gross=====

=====Gross=====

#Initially - hyperaemia of mucosa (e.g. catarrhal inflammation) then within 12 hours produces fluid filled vesicles on dorsum of [[Oral Cavity - Tongue - Anatomy & Physiology|tongue]], may be other places

#Initially - hyperaemia of mucosa (e.g. catarrhal inflammation) then within 12 hours produces fluid filled vesicles on dorsum of [[Tongue - Anatomy & Physiology|tongue]], may be other places

#Small vesicle coalesce to produce big ones -i.e. Bullae

#Small vesicle coalesce to produce big ones -i.e. Bullae

#Very quickly rupture; epithelium appears dirty grey in colour because of necrosis - sloughed skin, very good for diagnosis

#Very quickly rupture; epithelium appears dirty grey in colour because of necrosis - sloughed skin, very good for diagnosis