*The host's immune system needs to evoke an immune response against the antigen which can take a few days
*The host's immune system needs to evoke an immune response against the antigen which can take a few days
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*Can require two or more doses to be effective; the first dose initiates the '''priming''' reaction where [[Immunoglobulins|antibody]] production ceases after a few weeks, but the second and subsequent doses create [[B cell differentiation#Memory cells|memory cells]] which remain in the circulation for a much longer period of time
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*Can require two or more doses to be effective; the first dose initiates the '''priming''' reaction where antibody production ceases after a few weeks, but the second and subsequent doses create memory cells which remain in the circulation for a much longer period of time.