Changes

== Synchronization of Recipients with the Donor ==

== Synchronization of Recipients with the Donor ==

* GOAL: To synchronize the donor and recipient to be in the same stage of the [[Female_Reproductive_Tract_-Oestrous_Cycle_-_Anatomy_%26_Physiology|oestrous cycle]].

* GOAL: To synchronize the donor and recipient to be in the same stage of the [[Female_Reproductive_Tract_-Oestrous_Cycle_- Anatomy & Physiology|oestrous cycle]].

* REASON: To prepare the [[Female_Reproductive_Tract_-The_Uterus_-_Anatomy_%26_Physiology|uterus]] of the recipient to support [[Blastocyst Embryonic Development - Anatomy & Physiology|preattachment embryogenesis]].  

* REASON: To prepare the [[Female_Reproductive_Tract_-The_Uterus_- Anatomy & Physiology|uterus]] of the recipient to support [[Blastocyst Embryonic Development - Anatomy & Physiology|preattachment embryogenesis]].  

* HOW: Treat recipient with a hormonal regime that induces oestrus to occur at the same time as the donor.

* HOW: Treat recipient with a hormonal regime that induces oestrus to occur at the same time as the donor.

== Superovulation of the Donor ==

== Superovulation of the Donor ==

* GOAL: To hyperstimulate [[Female_Reproductive_Tract_-The_Ovary_-_Anatomy_%26_Physiology|ovaries]] with gonadotrophins.

* GOAL: To hyperstimulate [[Female_Reproductive_Tract_-The_Ovary_- Anatomy & Physiology|ovaries]] with gonadotrophins.

* REASON: To provide higher than normal numbers of [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicles]] that reach dominance and [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulate]].

* REASON: To provide higher than normal numbers of [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]] that reach dominance and [[The_Ovary_-_Ovulation_- Anatomy & Physiology|ovulate]].

* HOW: Inject donor with gonadotrophins to hyperstimulate follicular development.  Generally '''FSH''' or one of its analogs is used.

* HOW: Inject donor with gonadotrophins to hyperstimulate follicular development.  Generally '''FSH''' or one of its analogs is used.

== Inseminating the Donor ==

== Inseminating the Donor ==

* GOAL: Inseminate donor with semen from genetically superior bulls. To generate the best [[Fertilisation%2C_Implantation_and_Early_Embryonic_Development_-Fertilisation-_Anatomy_%26_Physiology|fertilisation]] rates and genetic combinations possible.       

* GOAL: Inseminate donor with semen from genetically superior bulls. To generate the best [[Fertilisation%2C_Implantation_and_Early_Embryonic_Development_-Fertilisation- Anatomy & Physiology|fertilisation]] rates and genetic combinations possible.       

* REASON: Enhance rate of genetic progress.

* REASON: Enhance rate of genetic progress.

* HOW: Use highly fertile semen and well-trained, experienced inseminators.

* HOW: Use highly fertile semen and well-trained, experienced inseminators.

* REASON: To recover viable embryos.

* REASON: To recover viable embryos.

* HOW:  

* HOW:  

** Before the procedure is started, a local anaesthetic is injected to cause relaxation of the [[Rectum_-_Anatomy_%26_Physiology|rectum]].   

** Before the procedure is started, a local anaesthetic is injected to cause relaxation of the [[Rectum_- Anatomy & Physiology|rectum]].   

** At day 6-8 a specialized catheter has a small balloon that can be inflated to prevent retrogade flow of the flushing medium.

** At day 6-8 a specialized catheter has a small balloon that can be inflated to prevent retrogade flow of the flushing medium.

** A flushing medium is then introduced into the [[Female_Reproductive_Tract_-The_Uterus_-_Anatomy_%26_Physiology|uterus]], lavaged and returned through the catheter to a collection vessel.

** A flushing medium is then introduced into the [[Female_Reproductive_Tract_-The_Uterus_- Anatomy & Physiology|uterus]], lavaged and returned through the catheter to a collection vessel.

* REASON: To achieve pregnancy in each recipient.

* REASON: To achieve pregnancy in each recipient.

* HOW:  

* HOW:  

** Both donor and recipient have a [[The_Ovary_-Corpus_Luteum_-_Anatomy_%26_Physiology|corpus luteum]] at similar stages of leutinization.

** Both donor and recipient have a [[The_Ovary_-Corpus_Luteum_- Anatomy & Physiology|corpus luteum]] at similar stages of leutinization.

** [[Female_Reproductive_Tract_-The_Uterus_-_Anatomy_%26_Physiology|Uterine environment]] in the donor and recipient are quite similar.

** [[Female_Reproductive_Tract_-The_Uterus_- Anatomy & Physiology|Uterine environment]] in the donor and recipient are quite similar.

** A single embryo is placed into the uterine horn using a transfer pipette.

** A single embryo is placed into the uterine horn using a transfer pipette.

[[Category:Reproductive System]]

[[Category:Reproductive System]]