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* Most spermatozoa produced are lost in the urine during periods of sexual abstinence.
 
* Most spermatozoa produced are lost in the urine during periods of sexual abstinence.
* The presence of sperm in the urine indicates that [[Male_Reproductive_Tract_-Spermatogenesis_and_Spermiation_- Anatomy & Physiology|spermatogenesis]] is occuring.
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* The presence of sperm in the urine indicates that [[Spermatogenesis_and_Spermiation_- Anatomy & Physiology|spermatogenesis]] is occuring.
 
* Frequent urine collection is difficult in large domestic species and requires special equipment, so this method of determining pubertal onset has limitations.
 
* Frequent urine collection is difficult in large domestic species and requires special equipment, so this method of determining pubertal onset has limitations.
  
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