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| ==<font color="purple">ELISA testing</font>== | | ==<font color="purple">ELISA testing</font>== |
− | {| border="3" cellpadding="8"
| + | <FlashCard questions="3"> |
− | !width="400"|'''Question'''
| + | |q1=What does the acronym ELISA stand for? |
− | !width="400"|'''Answer'''
| + | |a1=Enzyme Linked ImmunoSorbent Assay |
− | !width="150"|'''Article'''
| + | |l1=ELISA testing |
− | |-
| + | |q2=What type of ELISA requires all the reagents to be added at once? |
− | |<big>'''What does the acronym ELISA stand for?'''
| + | |a2=Homogenous |
− | ||<font color="white"> <big> | + | |l2=ELISA testing |
− | *'''''Enzyme Linked ImmunoSorbent Assay'''''
| + | |q3=What are the five basic steps of an ELISA test? |
− | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] | + | |a3= |
− | |-
| + | *Adsorption of antigen/antibody onto solid phase |
− | |<big>'''What type of ELISA requires all the reagents to be added at once?'''
| + | *Addition of chosen sample and reagents |
− | ||<font color="white"> <big> | + | *Incubation and washing |
− | *'''''Homogenous'''''
| + | *Addition of enzyme-labelled antigen/antibody |
− | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] | + | *Addition of specific substrate |
− | |-
| + | |l3=ELISA testing |
− | |<big>'''What are the five basic steps of an ELISA test?'''
| + | </FlashCard> |
− | ||<font color="white"> <big> | |
− | *'''''Adsorption of antigen/antibody onto solid phase''''' | |
− | *'''''Addition of chosen sample and reagents''''' | |
− | *'''''Incubation and washing''''' | |
− | *'''''Addition of enzyme-labelled antigen/antibody''''' | |
− | *'''''Addition of specific substrate''''' | |
− | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] | |
| | | |
− | |}
| |
| ==<font color="purple">Immunofluorescence</font>== | | ==<font color="purple">Immunofluorescence</font>== |
− | {| border="3" cellpadding="8"
| + | <FlashCard questions="4"> |
− | !width="400"|'''Question'''
| + | |q1=The adsorption of light by a molecule is known as what? |
− | !width="400"|'''Answer'''
| + | |a1=Excitation |
− | !width="150"|'''Article'''
| + | |l1=Immunofluorescence |
− | |-
| + | |q2=What are the fluorescent dyes commonly known as? |
− | |<big>'''The adsorption of light by a molecule is known as what?'''
| + | |a2=Fluorochromes |
− | ||<font color="white"> <big> | + | |l2=Immunofluorescence |
− | *'''''Excitation'''''
| + | |q3=What are the advantages of indirect staining? |
− | ||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]] | + | |a3= |
− | |-
| + | *Brighter fluorescence |
− | |<big>'''What are the fluorescent dyes commonly known as?'''
| + | *One preparation can be used to test many serum samples |
− | ||<font color="white"> <big> | + | *Relative expressions of different antigens in the same cell can be tested |
− | *'''''Fluorochromes'''''
| + | *Loss of antibody during conjugation is limited |
− | ||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]] | + | |l3=Immunofluorescence |
− | |-
| + | |q4=What technique is used to provide a quantitative measure of immunofluorescence? |
− | |<big>'''What are the advantages of indirect staining?'''
| + | |a4=Flow cytometry |
− | ||<font color="white"> <big> | + | |l4=Immunofluorescence |
− | *'''''Brighter fluorescence''''' | + | </FlashCard> |
− | *'''''One preparation can be used to test many serum samples''''' | |
− | *'''''Relative expressions of different antigens in the same cell can be tested''''' | |
− | *'''''Loss of antibody during conjugation is limited''''' | |
− | ||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]] | |
− | |-
| |
− | |<big>'''What technique is used to provide a quantitative measure of immunofluorescence?'''
| |
− | ||<font color="white"> <big> | |
− | *'''''Flow cytometry'''''
| |
− | ||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]] | |
− | |}
| |
| | | |
| ==<font color="purple">Radioimmunoassay</font>== | | ==<font color="purple">Radioimmunoassay</font>== |
− | {| border="3" cellpadding="8"
| + | <FlashCard questions="4"> |
− | !width="400"|'''Question'''
| + | |q1=What are radioimmunoassays commonly used to detect? |
− | !width="400"|'''Answer'''
| + | |a2= |
− | !width="150"|'''Article'''
| + | *Hormone levels in blood and tissue fluids |
− | |-
| + | *Serum proteins |
− | |<big>'''What are radioimmunoassays commonly used to detect?'''
| + | *Drugs |
− | ||<font color="white"> <big> | + | *Vitamins |
− | *'''''Hormone levels in blood and tissue fluids''''' | + | |l1=Radioimmunoassay |
− | *'''''Serum proteins''''' | + | |q2=What is the most common isotope used for radioimmunoassay? |
− | *'''''Drugs''''' | + | |a2=Iodine-125 |
− | *'''''Vitamins''''' | + | |l2=Radioimmunoassay |
− | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] | + | |q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? |
− | |-
| + | |a3=Ensures the number of epitopes present exceeds number of antibody-binding sites |
− | |<big>'''What is the most common isotope used for radioimmunoassay?'''
| + | |l3=Radioimmunoassay |
− | ||<font color="white"> <big> | + | |q4=What are the disadvantages of the radioimmunoassay? |
− | *'''''Iodine-125'''''
| + | |a4= |
− | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] | + | *Substances used are radioactive |
− | |-
| + | *Gamma radiation requires special counting equipment |
− | |<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?'''
| + | *Iodine naturally accumulates in the thyroid gland |
− | ||<font color="white"> <big> | + | |l4=Radioimmunoassay |
− | *'''''Ensures the number of epitopes present exceeds number of antibody-binding sites'''''
| + | </FlashCard> |
− | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] | |
− | |-
| |
− | |<big>'''What are the disadvantages of the radioimmunoassay?'''
| |
− | ||<font color="white"> <big> | |
− | *'''''Substances used are radioactive''''' | |
− | *'''''Gamma radiation requires special counting equipment''''' | |
− | *'''''Iodine naturally accumulates in the thyroid gland''''' | |
− | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] | |
| | | |
− | |}
| |
| ==<font color="purple">Agglutination</font>== | | ==<font color="purple">Agglutination</font>== |
− | {| border="3" cellpadding="8"
| + | <FlashCard questions="5"> |
− | !width="400"|'''Question'''
| + | |q1=What are the antibodies that produce agglutination known as? |
− | !width="400"|'''Answer'''
| + | |a1=Agglutinins |
− | !width="150"|'''Article'''
| + | |l1=Agglutination |
− | |-
| + | |q2=What constitutes the end of the agglutination test? |
− | |<big>'''What are the antibodies that produce agglutination known as?'''
| + | |a2=The last tube showing distinct agglutination |
− | ||<font color="white"> <big> | + | |l2=Agglutination |
− | *'''''Agglutinins'''''
| + | |q3=What does the titre represent? |
− | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | + | |a3=The number of antibodies per unit volume of serum |
− | |-
| + | |l3=Agglutination |
− | |<big>'''What constitutes the end of the agglutination test?'''
| + | |q4=What is the prozone effect? |
− | ||<font color="white"> <big> | + | |a4=An inhibition of agglutination caused by an excess of antibodies |
− | *'''''The last tube showing distinct agglutination'''''
| + | |l4=Agglutination |
− | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | + | |q5=What agglutination test is often used to test for rhesus disease? |
− | |-
| + | |a5=Coombs test |
− | |<big>'''What does the titre represent?'''
| + | |l5=Agglutination |
− | ||<font color="white"> <big> | + | </FlashCard> |
− | *'''''The number of antibodies per unit volume of serum'''''
| |
− | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | |
− | |-
| |
− | |<big>'''What is the prozone effect?'''
| |
− | ||<font color="white"> <big> | |
− | *'''''An inhibition of agglutination caused by an excess of antibodies'''''
| |
− | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | |
− | |-
| |
− | |<big>'''What agglutination test is often used to test for rhesus disease?'''
| |
− | ||<font color="white"> <big> | |
− | *'''''Coombs test'''''
| |
− | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | |
| | | |
| + | |
| + | <br><br> |
| + | {{Jim Bee 2007}} |
| [[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]] | | [[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]] |