Immunological Testing Flashcards
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ELISA testing
| Question | Answer | Article | |
| What does the acronym ELISA stand for? | Enzyme Linked ImmunoSorbent Assay
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Link to Article | |
| What type of ELISA requires all the reagents to be added at once? | Homogenous
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| What are the five basic steps of an ELISA test? |
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Immunofluorescence
| Question | Answer | Article | |
| The adsorption of light by a molecule is known as what? | Excitation
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Link to Article | |
| What are the fluorescent dyes commonly known as? | Fluorochromes
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Link to Article | |
| What are the advantages of indirect staining? |
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| What technique is used to provide a quantitative measure of immunofluorescence? | Flow cytometry
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Link to Article | |
Radioimmunoassay
| Question | Answer | Article | |
| What are radioimmunoassays commonly used to detect? | Link to Article | ||
| What is the most common isotope used for radioimmunoassay? | Iodine-125
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Link to Article | |
| Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? | Ensures the number of epitopes present exceeds number of antibody-binding sites
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Link to Article | |
| What are the disadvantages of the radioimmunoassay? |
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Agglutination
| Question | Answer | Article | |
| What are the antibodies that produce agglutination known as? | Agglutinins
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Link to Article | |
| What constitutes the end of the agglutination test? | The last tube showing distinct agglutination
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| What does the titre represent? | The number of antibodies per unit volume of serum
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Link to Article | |
| What is the prozone effect? | An inhibition of agglutination caused by an excess of antibodies
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Link to Article | |
| What agglutination test is often used to test for rhesus disease? | Coombs test
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Originally funded by the RVC Jim Bee Award 2007 |