Difference between revisions of "Embryo Transfer - Anatomy & Physiology"

Revision as of 14:12, 16 July 2008

BACK TO REPRODUCTIVE SYSTEM BACK TO REPRODUCTIVE TECHNOLOGIES

Introduction

Synchronization of Recipients with the Donor

GOAL: To synchronize the donor and recipient to be in the same stage of the oestrous cycle.
REASON: To prepare the uterus of the recipient to support preattachment embryogenesis.
HOW: Treat recipient with a hormonal regime that induces oestrus to occur at the same time as the donor.

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+<big><center>[[Reproductive System|'''BACK TO REPRODUCTIVE SYSTEM''']]</center></big>
+<big><center>[[Reproductive_System#Reproductive_Technologies|'''BACK TO REPRODUCTIVE TECHNOLOGIES''']]</center></big>
 == Introduction ==
-Multiple ovulation and embryo transfer has been commercially available since the 1970s, but not widely used because it requires a highly skilled team.
 == Synchronization of Recipients with the Donor ==
-* GOAL: To synchronize the donor and recipient to be in the same stage of the [[Oestrous Cycle - Anatomy & Physiology|oestrous cycle]].
+* GOAL: To synchronize the donor and recipient to be in the same stage of the oestrous cycle.
-* REASON: To prepare the [[Uterus - Anatomy & Physiology|uterus]] of the recipient to support [[Blastocyst Embryonic Development - Anatomy & Physiology|preattachment embryogenesis]].
+* REASON: To prepare the uterus of the recipient to support preattachment embryogenesis.
 * HOW: Treat recipient with a hormonal regime that induces oestrus to occur at the same time as the donor.
 == Superovulation of the Donor ==
-* GOAL: To hyperstimulate [[Ovary - Anatomy & Physiology|ovaries]] with gonadotrophins.
-* REASON: To provide higher than normal numbers of [[Follicles - Anatomy & Physiology|follicles]] that reach dominance and [[Ovulation - Anatomy & Physiology|ovulate]].
-* HOW: Inject donor with gonadotrophins to hyperstimulate follicular development.  Generally '''FSH''' or one of its analogs is used.
 == Inseminating the Donor ==
-* GOAL: Inseminate donor with semen from genetically superior bulls. To generate the best [[Fertilisation - Anatomy & Physiology|fertilisation]] rates and genetic combinations possible.
-* REASON: Enhance rate of genetic progress.
-* HOW: Use highly fertile semen and well-trained, experienced inseminators.
 == Recovery and Identification of Viable Embryos ==
-* GOAL: To nonsurgically collect (flush) embryos from the donor to transfer.
-* REASON: To recover viable embryos.
-* HOW:
-** Before the procedure is started, a local anaesthetic is injected to cause relaxation of the [[Rectum_- Anatomy & Physiology|rectum]].
-** At day 6-8 a specialized catheter has a small balloon that can be inflated to prevent retrogade flow of the flushing medium.
-** A flushing medium is then introduced into the [[Uterus - Anatomy & Physiology|uterus]], lavaged and returned through the catheter to a collection vessel.
 == Transfer of Viable Embryos into Synchronised Recipients ==
-* GOAL: To deposit a potentially viable embryo into the uterine horn of each recipient.
-* REASON: To achieve pregnancy in each recipient.
-* HOW:
-** Both donor and recipient have a [[Corpus Luteum - Anatomy & Physiology|corpus luteum]] at similar stages of leutinization.
-** [[Uterus - Anatomy & Physiology|Uterine environment]] in the donor and recipient are quite similar.
-** A single embryo is placed into the uterine horn using a transfer pipette.
-[[Category:Reproductive Technologies]]
-[[Category:Bullet Points]]

Difference between revisions of "Embryo Transfer - Anatomy & Physiology"

Revision as of 14:12, 16 July 2008

Contents

Introduction

Synchronization of Recipients with the Donor

Superovulation of the Donor

Inseminating the Donor

Recovery and Identification of Viable Embryos

Transfer of Viable Embryos into Synchronised Recipients

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