Difference between revisions of "Immunodiffusion"
Jump to navigation
Jump to search
(New page: Immunodiffusion reactions are diagnostic tests that use the diffusion of particles through a medium (such as agar) to measure: *relative concentrations of antibodies/antigens *relative pur...) |
|||
| Line 5: | Line 5: | ||
There are two types of immunodiffusion reaction, both being carried out on a semisolid medium: | There are two types of immunodiffusion reaction, both being carried out on a semisolid medium: | ||
*'''radial immunodiffusion''': also known as the Mancini method. The medium is prepared with a suitable dilution of antiserum and the antigen sample placed on top and allowed to diffuse. As diffusion takes place, large insoluble complexes form creating a ring of precipitation (precipitin). By comparing the area of the ring with a standard curve, the concentration of the antigen sample can be determined. | *'''radial immunodiffusion''': also known as the Mancini method. The medium is prepared with a suitable dilution of antiserum and the antigen sample placed on top and allowed to diffuse. As diffusion takes place, large insoluble complexes form creating a ring of precipitation (precipitin). By comparing the area of the ring with a standard curve, the concentration of the antigen sample can be determined. | ||
| − | *'''double immunodiffusion'''also known as the Ouchterlony method. Antigen and antibody preparations are placed on the medium and both allowed to diffuse radially from the wells towards each other. This establishes a concentration gradient, forming an area of equivalence and line of precipitin. | + | *'''double immunodiffusion''': also known as the Ouchterlony method. Antigen and antibody preparations are placed on the medium and both allowed to diffuse radially from the wells towards each other. This establishes a concentration gradient, forming an area of equivalence and line of precipitin. |
Revision as of 12:50, 11 September 2008
Immunodiffusion reactions are diagnostic tests that use the diffusion of particles through a medium (such as agar) to measure:
- relative concentrations of antibodies/antigens
- relative purity of an antigen preparation
There are two types of immunodiffusion reaction, both being carried out on a semisolid medium:
- radial immunodiffusion: also known as the Mancini method. The medium is prepared with a suitable dilution of antiserum and the antigen sample placed on top and allowed to diffuse. As diffusion takes place, large insoluble complexes form creating a ring of precipitation (precipitin). By comparing the area of the ring with a standard curve, the concentration of the antigen sample can be determined.
- double immunodiffusion: also known as the Ouchterlony method. Antigen and antibody preparations are placed on the medium and both allowed to diffuse radially from the wells towards each other. This establishes a concentration gradient, forming an area of equivalence and line of precipitin.