Difference between revisions of "Chlamydia Species - General Characteristics"

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==Introduction==
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===Overview===
Chlamydia species include host adapted and non-host adapted species varying in virulence for different hosts. They can casue various diseases, including respiratory, enteric, pleural and reproductive diseases in animals and humans; specific conditions include conjunctivitis, arthritis, abortion, urethritis, enteritis, pneumonia, encephalomyelitis. Disease manifestation varies from subclinical to severe systemic infections - intestinal infections are often subclinical and persistent.Human infections are commonly acquired from infected birds with psittacosis or ornithosis, causing respiratory infections in the handler.
 
  
 +
*Host adapted and non-host adapted species varying in virulence for different hosts
 +
*Species cause specific diseases in particular hosts
 +
*Respiratory, enteric, pleural and reproductive diseases in animals and humans
 +
*Conjunctivitis, arthritis, abortion, urethritis, enteritis, pneumonia, encephalomyelitis
 +
*Manifestation varies from subclinical to severe systemic infections
 +
*Intestinal infections often subclinical and persistent
 +
*Human infections usualy acquired from infected birds, causing psittacosis or ornithosis, causing respiratory infections
  
==Bacterial Characteristics==
 
Chlamydia species are obligate intracellular Gram negative bacteria with ribosomes, DNA and RNA and an outer membrane containing lipopolysaccharides (LPS). The peptidoglycan cell wall is resistant to lysozymes.
 
  
These bacteria only grow in the presence of living eukaryotic cells as they are unable to synthesise ATP therefore requiring intermediates from host cells. There are two morphological forms:
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===Characteristics===
*Elementary body, the infective extracellular form, which is small, metabolically inert and osmotically stable; surrounded by cytoplasmic membrane, outer membrane with LPS, but no peptidoglycan
 
*Reticulate body: larger, metabolically active, osmotically fragile
 
The elementary body survives in the environment for several days.
 
  
==Pathogenesis and Pathogenicity==
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*Obligate intracellular bacteria
The elementary body enters the host epithelial cells by receptor-mediated endocytosis. It is subsequently transformation into a larger reticulate body within endosome known as an '''inclusion''', which divides by binary fission to form many new chlamydia cells. The reticulate bodies mature and condense to form elementary bodies which are released from the dying host cells approximately 72 hours after infection to infect other cells. Persistent infections can occur if replication is delayed by environmental conditions such as the presence of interferon gamma.
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*Gram negative bacteria with outer membrane, LPS, ribosomes, DNA and RNA
 +
*Peptidoglycan cell wall resistant to lysozyme
 +
*Only grow in presence of living eukaryotic cells
 +
*Unable to synthesis ATP therefore require intermediates from host cells
 +
*Not stained by Gram stain
 +
*Two morphological forms
 +
**Elementary body, the infective extracellular form, which is small, metabolically inert and osmotically stable; surrounded by cytoplasmic membrane, outer membrane with LPS, but no peptidoglycan
 +
**Retiuculate body: larger, metabolically active, osmotically fragile
 +
*Elementary body survives in the environment for several days
  
Many infections are subclinical due to the intracellular existence of ''chlamydia'' which avoids the body's inflammatory response. Chronic infections  may fail to induce any immune response, or may repeatedly stimulate the immune system, causing a delayed hypersensitivity reaction and tissue damage. Prolonged faecal shedding of organisms is a common sequelae. Clinical signs of infection depend on the route of infection and degree of exposure.
 
  
Clinical infections can occur in non-natural host species.
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===Pathogenesis and pathogenicity===
 +
 
 +
*Elementary body enters host epithelial cells by receptor-mediated endocytosis
 +
*Transformation into larger reticulate body within endosome; known as an inclusion
 +
*Reticulate body divides by binary fission to form many new chlamydia cells
 +
*Reticulate bodies mature and condense to form elementary bodies
 +
*Elementary bodies released from dying host cells after about 72 hours to infect other cells
 +
*Persistent infections can occur if replication delayed by environmental conditions such as presence of interferon gamma
 +
*Many infections subclinical due to intracellular existence of ''chlamydia'' preventing inflammatory reactions
 +
*Chronic infections  may fail to induce an immune response, or may repeatedly stimulate the immune system, causing a delayed hypersensitivity reaction and tissue damage
 +
*Prolonged faecal shedding of organisms
 +
*Clinical infections occur in non-natural host species
 +
*CLinical signs depend on route of infection and degree of exposure
 +
 
 +
===Diagnosis===
  
==Diagnosis of Infection==
 
 
*Direct microscopy of smears and tissues e.g. organs from aborted foetuses, liver/spleen from avian cases
 
*Direct microscopy of smears and tissues e.g. organs from aborted foetuses, liver/spleen from avian cases
 
*Kosters (modified Ziehl-Neelsen) stain of placental smears shows small red rods
 
*Kosters (modified Ziehl-Neelsen) stain of placental smears shows small red rods
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*Serological tests: complement fixation, ELISA, indirect immunofluorescence
 
*Serological tests: complement fixation, ELISA, indirect immunofluorescence
  
==Species Specific Infections==
 
===Sheep===
 
*Sporadic or outbreaks of lamb polyarthritis
 
*High morbidity, low mortality
 
*Commonly occurs together with conjunctivitis
 
*Most recover but may remain chronically lame
 
  
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*May cause [[Joints Inflammatory - Pathology#In Sheep|arthritis in sheep]] and [[Joints Inflammatory - Pathology#In Cattle|arthritis in cattle]]
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 +
'''sheep'''
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*[[:Category:Chlamydophila species|'''''Chlamydia sp.''''']]
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**Sporadic or outbreaks of lamb polyarthritis
 +
**High morbidity, low mortality
 +
**Commonly together with conjunctivitis
 +
**Most recover but may remain lame
 +
 +
 +
'''cattle'''
  
===Cattle===
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**[[:Category:Chlamydophila species|'''''Chlamydia sp.''''']]
*Severe disease in young calves
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***Severe disease in young calves
*High mortality
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***High mortality
*Can be seen in smears of synovial fluid from swollen joints
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***Can be seen in smears of synovial fluid from swollen joints
*Oedematous and hyperaemic surrounding tissue
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***Oedematous and hyperaemic surrounding tissue
*Possibly due to intrauterine infection
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***Possibly due to intrauterine infection
  
  
 
[[Category:Chlamydophila species]]
 
[[Category:Chlamydophila species]]
==Webinars==
 
<rss max="10" highlight="none">https://www.thewebinarvet.com/infection-control-and-biosecurity/webinars/feed</rss>
 

Revision as of 20:56, 5 November 2010

Overview

  • Host adapted and non-host adapted species varying in virulence for different hosts
  • Species cause specific diseases in particular hosts
  • Respiratory, enteric, pleural and reproductive diseases in animals and humans
  • Conjunctivitis, arthritis, abortion, urethritis, enteritis, pneumonia, encephalomyelitis
  • Manifestation varies from subclinical to severe systemic infections
  • Intestinal infections often subclinical and persistent
  • Human infections usualy acquired from infected birds, causing psittacosis or ornithosis, causing respiratory infections


Characteristics

  • Obligate intracellular bacteria
  • Gram negative bacteria with outer membrane, LPS, ribosomes, DNA and RNA
  • Peptidoglycan cell wall resistant to lysozyme
  • Only grow in presence of living eukaryotic cells
  • Unable to synthesis ATP therefore require intermediates from host cells
  • Not stained by Gram stain
  • Two morphological forms
    • Elementary body, the infective extracellular form, which is small, metabolically inert and osmotically stable; surrounded by cytoplasmic membrane, outer membrane with LPS, but no peptidoglycan
    • Retiuculate body: larger, metabolically active, osmotically fragile
  • Elementary body survives in the environment for several days


Pathogenesis and pathogenicity

  • Elementary body enters host epithelial cells by receptor-mediated endocytosis
  • Transformation into larger reticulate body within endosome; known as an inclusion
  • Reticulate body divides by binary fission to form many new chlamydia cells
  • Reticulate bodies mature and condense to form elementary bodies
  • Elementary bodies released from dying host cells after about 72 hours to infect other cells
  • Persistent infections can occur if replication delayed by environmental conditions such as presence of interferon gamma
  • Many infections subclinical due to intracellular existence of chlamydia preventing inflammatory reactions
  • Chronic infections may fail to induce an immune response, or may repeatedly stimulate the immune system, causing a delayed hypersensitivity reaction and tissue damage
  • Prolonged faecal shedding of organisms
  • Clinical infections occur in non-natural host species
  • CLinical signs depend on route of infection and degree of exposure

Diagnosis

  • Direct microscopy of smears and tissues e.g. organs from aborted foetuses, liver/spleen from avian cases
  • Kosters (modified Ziehl-Neelsen) stain of placental smears shows small red rods
  • Blue inclusions in cytoplasm of Giemsa-stained cells
  • Methylene blue stain with darkfield microscopy
  • Fluorescent antibody stain
  • Antigen detection kits for diagnosis from swabs
  • ELISA to detect Chlamydophila LPS
  • Isolation in embryonated eggs and McCoy cells as well as animal tissues
  • PCR to detect chlamydial DNA
  • Serological tests: complement fixation, ELISA, indirect immunofluorescence


sheep

  • Chlamydia sp.
    • Sporadic or outbreaks of lamb polyarthritis
    • High morbidity, low mortality
    • Commonly together with conjunctivitis
    • Most recover but may remain lame


cattle

    • Chlamydia sp.
      • Severe disease in young calves
      • High mortality
      • Can be seen in smears of synovial fluid from swollen joints
      • Oedematous and hyperaemic surrounding tissue
      • Possibly due to intrauterine infection