Difference between revisions of "Intracytoplasmic Sperm Injection - Anatomy & Physiology"

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|linkpage =Reproductive System - Anatomy & Physiology
 
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* Common in human, effective for laboratory mice.
 
* Common in human, effective for laboratory mice.
 
* Sperm need not be [[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology#Capacitation|capacitated]], they can be epididymal,testicular or even a spermatid.
 
* Sperm need not be [[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology#Capacitation|capacitated]], they can be epididymal,testicular or even a spermatid.
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Revision as of 18:09, 1 December 2010

  • Common in human, effective for laboratory mice.
  • Sperm need not be capacitated, they can be epididymal,testicular or even a spermatid.
    • Only requirement is genes and cytoplasm
    • Even dead freeze-dried sperm have been effective in mice.
  • Abnormalities are frequent.
  • Power is in the hands of the operator, as it bypasses the process of selection in the female tract.
    • Operator must select a single sperm to inject directly into the Oocyte under a microscope.
  • Oocyte then placed in cell culture for development.