Difference between revisions of "Bacillus species"

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===''Bacillus anthracis''===
 
===''Bacillus anthracis''===
  
*
+
*Epidemiology:
 +
**Endospore formation allows persistence and spread
 +
**Endospores survive decades in the soil
 +
**Outbreaks in herbivores grazing pastures contaminated by spores from buried carcases
 +
**Infection usually by ingestion of spores
 
*Pathogenesis and pathogenicity:
 
*Pathogenesis and pathogenicity:
 
**Saprophyte in soil
 
**Saprophyte in soil
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**Protective factor binds to the cell receptor to allow action of the other factors
 
**Protective factor binds to the cell receptor to allow action of the other factors
 
**The toxin kills phagocytes, increases capillary permeability and interferes with clooting cascade
 
**The toxin kills phagocytes, increases capillary permeability and interferes with clooting cascade
**Capillary thrombosis; leakage of fluid through damaged capillary endothelium
 
**Systemic shock from circulatory collapse, haemorrhage and oedema lead to death of the animal
 
 
*Clinical signs:
 
*Clinical signs:
**
+
**Cattle:
 +
***Fatal peracute septicaemia
 +
***Capillary thrombosis; leakage of fluid through damaged capillary endothelium
 +
***Systemic shock from circulatory collapse, haemorrhage and oedema lead to death of the animal
 +
**Pigs:
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***Subacute anthrax with oedematous pharyngeal swelling; intestinal form with high mortality
 +
**Horses:
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***Subacute anthrax with localised oedema; septicaemia with colic and enteritis
  
  
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*Associated with food spoilage
 
*Associated with food spoilage
 
*Abortion in cattle and sheep, possibly from spoiled silage or hay
 
*Abortion in cattle and sheep, possibly from spoiled silage or hay
 +
 +
 +
===''Bacillus cereus''===
 +
 +
*Mastitis in cattle
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*Food poisoning and eye infections in humans

Revision as of 13:01, 17 May 2008

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Overview

  • Mostly non-pathogenic environmental organisms
  • Bacillus anthracis causes anthrax
  • Anthrax is a severe disease affecting all mammals worldwide
  • Ruminants are highly susceptible to anthrax, dying of a septicaemic form
  • Pigs and horses are moderately susceptible, but carnivores are fairly resistant
  • Bacillus licheniformis may cause shoradic abortions in cattle and sheep
  • Bacillus cereus causes food poisoning in humans and mastitis in cows


Characteristics

  • Large, Gram positive rods
  • Produce endospores
  • Aerobes or facultative anaerobes
  • Grow on non-enriched media
  • Motile
  • Catalase positive, oxidase negative
  • Bacillus anthracis colonies are up to 5mm diameter, flat, dry, grey, with a ground-glass appearance; curled outgrowthsfrom the edge of the colony give a 'medusa head' appearance; non-haemolytic (differentiate from Bacillus cereus)
  • Bacillus licheniformis forms dull, rough, wrinkled colonies, with hair-like outgrowths
  • Biochemical tests for identification
  • Can often tolerate adverse environmental conditions


Bacillus anthracis

  • Epidemiology:
    • Endospore formation allows persistence and spread
    • Endospores survive decades in the soil
    • Outbreaks in herbivores grazing pastures contaminated by spores from buried carcases
    • Infection usually by ingestion of spores
  • Pathogenesis and pathogenicity:
    • Saprophyte in soil
    • Capsule composed of homopolymer of D-isomer of glutamic acid allows survival in the body by resisting phagocytosis
    • Capsule stains mauve with polychrome methylene blue in the MacFadyean's reaction - identify anthrax in blood samples
    • Bacilli appear as chains of dark blue, square-ended rods surounded with the capsule
    • Extracellular toxin (holotoxin) composed of oedema factor, protective factor and lethal factor
    • Oedema factor is an adenylate cyclase which increases intracellular cAMP concetrations, damaging phagocytes
    • Protective factor binds to the cell receptor to allow action of the other factors
    • The toxin kills phagocytes, increases capillary permeability and interferes with clooting cascade
  • Clinical signs:
    • Cattle:
      • Fatal peracute septicaemia
      • Capillary thrombosis; leakage of fluid through damaged capillary endothelium
      • Systemic shock from circulatory collapse, haemorrhage and oedema lead to death of the animal
    • Pigs:
      • Subacute anthrax with oedematous pharyngeal swelling; intestinal form with high mortality
    • Horses:
      • Subacute anthrax with localised oedema; septicaemia with colic and enteritis


Bacillus licheniformis

  • Widespread in the environment
  • Associated with food spoilage
  • Abortion in cattle and sheep, possibly from spoiled silage or hay


Bacillus cereus

  • Mastitis in cattle
  • Food poisoning and eye infections in humans