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5 bytes added ,  10:01, 11 September 2008
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# Antibody is adsorbed onto solid phase
 
# Antibody is adsorbed onto solid phase
 
# Wash
 
# Wash
# Sample is added- specific antigen binds to antibody
+
# Sample serum is added- specific antigen binds to antibody
 
# Wash
 
# Wash
 
# Enzyme-labeled specific antibody is added- attaches to bound antigen
 
# Enzyme-labeled specific antibody is added- attaches to bound antigen
 
# Wash
 
# Wash
 
# Enzyme substrate is added
 
# Enzyme substrate is added
''visualised product = amount of antigen in the sample''
+
''visualised product = amount of antigen in the serum''
    
'''Antibody Class Capture Assay''' (for antibody detection)
 
'''Antibody Class Capture Assay''' (for antibody detection)
 
# Class-specific antiglobulin is adsorbed onto solid phase
 
# Class-specific antiglobulin is adsorbed onto solid phase
 
# Wash
 
# Wash
# Sample is added- class-specific antibody in the sample binds to antiglobulin
+
# Sample serum is added- class-specific antibody in the serum binds to antiglobulin
 
# Wash
 
# Wash
 
# Antigen is added- attaches to specific antibody  
 
# Antigen is added- attaches to specific antibody  
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# Wash
 
# Wash
 
# Enzyme substrate is added
 
# Enzyme substrate is added
''visualised product = amount of specific antibody in sample''
+
''visualised product = amount of specific antibody in serum''
    
'''Indirect Method''' (for antibody detection)
 
'''Indirect Method''' (for antibody detection)
 
# Antigen is adsorbed onto solid phase
 
# Antigen is adsorbed onto solid phase
 
# Wash
 
# Wash
# Sample is added- any specific antibody present in sample binds to antigen
+
# Serum is added- any specific antibody present in sample binds to antigen
 
# Wash
 
# Wash
 
# Enzyme-labeled antiglobulin is added- attaches to antibody
 
# Enzyme-labeled antiglobulin is added- attaches to antibody
 
# Wash
 
# Wash
 
# Enzyme substrate is added
 
# Enzyme substrate is added
''visualised product = amount of antibody in the sample''
+
''visualised product = amount of antibody in the serum''
    
===Competitive ELISA===
 
===Competitive ELISA===
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# Antigen is adsorbed onto solid phase
 
# Antigen is adsorbed onto solid phase
 
# Wash
 
# Wash
# Enzyme-labeled antibody (pre-titrated- optimal colour development) and sample are added
+
# Enzyme-labeled antibody (pre-titrated- optimal colour development) and serum are added
 
# Wash
 
# Wash
 
# Enzyme substrate is added
 
# Enzyme substrate is added
Line 101: Line 101:  
# Antigen is adsorbed onto solid phase
 
# Antigen is adsorbed onto solid phase
 
# Wash
 
# Wash
# Antigen in sample is incubated with enzyme-labelled antibody (again pre-titrated): this is directed against the antigen on the solid phase
+
# Antigen in serum is incubated with enzyme-labelled antibody (again pre-titrated): this is directed against the antigen on the solid phase
 
# Wash
 
# Wash
 
# Enzyme substrate is added
 
# Enzyme substrate is added
 
''visualised product = amount of enzyme-labelled antigen''
 
''visualised product = amount of enzyme-labelled antigen''
178

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