Changes

== Oocyte Collection==

== Oocyte Collection==

* Donor is superovulated to provide large numbers of mature [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]].

* Donor is superovulated to provide large numbers of mature [[Follicles - Anatomy & Physiology|follicles]].

* Pre-ovulatory [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]] are collected by a surgical procedure involving aspiration of the follicular content.

* Pre-ovulatory [[Follicles - Anatomy & Physiology|follicles]] are collected by a surgical procedure involving aspiration of the follicular content.

* [[Oogenesis - Anatomy & Physiology|Oocytes]] captured in vitro.

* [[Oogenesis - Anatomy & Physiology|Oocytes]] captured in vitro.

* Direct [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]] aspiration:  

* Direct [[Follicles - Anatomy & Physiology|follicle]] aspiration:  

** A hyperdermic needle is inserted into the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]].

** A hyperdermic needle is inserted into the [[Follicles - Anatomy & Physiology|follicle]].

** Follicular fluid is aspirated.

** Follicular fluid is aspirated.

** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]].

** Follicular fluid forcefully returned to the [[Follicles - Anatomy & Physiology|follicle]].

** Repeated 2-3 times to dislodge [[Oogenesis - Anatomy & Physiology|oocytes]].

** Repeated 2-3 times to dislodge [[Oogenesis - Anatomy & Physiology|oocytes]].

** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_- Anatomy & Physiology|rectum]].

** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_- Anatomy & Physiology|rectum]].

** Lubricated ultrasound transducer is inserted into the [[Vagina and Vestibule - Anatomy & Physiology|vagina]] and held in the fornix vagina.

** Lubricated ultrasound transducer is inserted into the [[Vagina and Vestibule - Anatomy & Physiology|vagina]] and held in the fornix vagina.

** [[Ovary - Anatomy & Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]] can be visualized.

** [[Ovary - Anatomy & Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[Follicles - Anatomy & Physiology|follicle]] can be visualized.

** Hypodermic needle is advanced through the [[Vagina and Vestibule - Anatomy & Physiology|vaginal wall]] into the antral follicle.

** Hypodermic needle is advanced through the [[Vagina and Vestibule - Anatomy & Physiology|vaginal wall]] into the antral follicle.

** Follicular fluid containing the [[Oogenesis - Anatomy & Physiology|oocyte]] is aspirated under constant vacuum.

** Follicular fluid containing the [[Oogenesis - Anatomy & Physiology|oocyte]] is aspirated under constant vacuum.