Difference between revisions of "Lizard Necropsy"

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*Examine all organs in a systematic manner - heart, great vessels, lung, thyroid, liver, gall bladder, spleen, pancreas, oesophagus, stomach, small intestine, large intestine, adrenal glands, kidneys, gonads.  
 
*Examine all organs in a systematic manner - heart, great vessels, lung, thyroid, liver, gall bladder, spleen, pancreas, oesophagus, stomach, small intestine, large intestine, adrenal glands, kidneys, gonads.  
 
*Open trachea, lungs, heart, oesophagus, stomach, and the intestinal tract and section others.  
 
*Open trachea, lungs, heart, oesophagus, stomach, and the intestinal tract and section others.  
 +
*Take swabs as appropriate including heart blood, liver, spleen and kidney.
 
*Note the colour, size, shape and any abnormalities of each organ.  
 
*Note the colour, size, shape and any abnormalities of each organ.  
*Take samples for culture and histopathology from diseased tissues and collect faeces from the intestinal tract for parasites and culture
+
*Take sterile smear of heart blood for staining.
 +
*Put liver, lung, kidney, spleen and intestine in both formal saline (transfer to 70% ethanol after 48 hours) and frozen in sterile container.
 +
*Make impression smears of different parts of intestines.
 +
*Take a sterile sample of any abnormal gut contents, make a smear in saline.
 +
*Wash the gut contents out in saline, examine for signs of parasites.
 +
*Inspect the gonads to sex definitively.
  
 
[[Category:Lizard_Examination|N]]
 
[[Category:Lizard_Examination|N]]

Revision as of 18:26, 25 March 2010



It is important that post mortem examinations are carried out with a systematic approach.

External examination

  • Measure and weigh the lizard and examine the external features.
  • Check the skin for bruising, bleeding, ulcerations, burns, lumps, scars, retained shed and ectoparasites.
  • Examine the nares, eyes, tympanic membranes, oral cavity and cloaca.
  • Assess the musculoskeletal system for body condition and palpate for pathology of the bones

Internal examination

  • Open the coelomic cavity. Check for fluid.
  • Examine all organs in a systematic manner - heart, great vessels, lung, thyroid, liver, gall bladder, spleen, pancreas, oesophagus, stomach, small intestine, large intestine, adrenal glands, kidneys, gonads.
  • Open trachea, lungs, heart, oesophagus, stomach, and the intestinal tract and section others.
  • Take swabs as appropriate including heart blood, liver, spleen and kidney.
  • Note the colour, size, shape and any abnormalities of each organ.
  • Take sterile smear of heart blood for staining.
  • Put liver, lung, kidney, spleen and intestine in both formal saline (transfer to 70% ethanol after 48 hours) and frozen in sterile container.
  • Make impression smears of different parts of intestines.
  • Take a sterile sample of any abnormal gut contents, make a smear in saline.
  • Wash the gut contents out in saline, examine for signs of parasites.
  • Inspect the gonads to sex definitively.