Immunological Testing Flashcards

IMMUNOLOGICAL TESTING WIKIBLOOD FLASHCARDS

ELISA testing

Question	Answer	Article
What does the acronym ELISA stand for?	Enzyme Linked ImmunoSorbent Assay	Link to Answer Article
What type of ELISA requires all the reagents to be added at once?	Homogenous	Link to Answer Article
What are the five basic steps of an ELISA test?	Adsorption of antigen/antibody onto solid phase Addition of chosen sample and reagents Incubation and washing Addition of enzyme-labelled antigen/antibody Addition of specific substrate	Link to Answer Article

Immunofluorescence

Question	Answer	Article
The adsorption of light by a molecule is known as what?	Excitation	Link to Answer Article
What are the fluorescent dyes commonly known as?	Fluorochromes	Link to Answer Article
What are the advantages of indirect staining?	Brighter fluorescence One preparation can be used to test many serum samples Relative expressions of different antigens in the same cell can be tested Loss of antibody during conjugation is limited	Link to Answer Article
What technique is used to provide a quantitative measure of immunofluorescence?	Flow cytometry	Link to Answer Article