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Clostridium species (view source)

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===Pathogenesis and pathogenicity===

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*''C. perfringens'' colonies are surrounded by a zone of double haemolysis

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* ''Clostridium tetani'' may infect wounds, giving rise to [[Tremors and Movement Disorders - Nervous System#Tetanus|tetanus]]

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===Diagnosis===

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*Anaerobic transport medium

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*Culture on blood agar enriched with yeast extract, vitamin K and haemin

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*Anaerobic culture with hydrogen supplement and 5-10% carbon dioxide

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*''C. perfringens'' colonies are surrounded by a zone of double haemolysis

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*Biochemical tests

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*Toxins identified in body fluids by toxin neutralisation or protection tests in lab animals

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*Fluorescent antibody tests for histotoxic clostridia

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*ELISA, PCR for toxin detection

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