Changes

ELISA testing (view source)

60 bytes added , 10:03, 11 September 2008

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# Enzyme-labeled specific antibody is added- attaches to bound antigen

# Wash

−

# Enzyme substrate is added

+

# Enzyme substrate is added (with dye for visualisation)

''visualised product = amount of antigen in the serum''

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'''Indirect Method''' (for antibody detection)

−

# ~~Antigen~~ is adsorbed onto solid phase

+

# Specific antigen (i.e. not from sample) is adsorbed onto solid phase

# Wash

# Serum is added- any specific antibody present in sample binds to antigen

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# Wash

# Enzyme substrate is added

−

''visualised product = amount of ~~enzyme-labelled antibody~~''

+

''visualised product = amount of antigen in serum sample''

'''Direct Antigen Competition''' (for antigen detection)

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