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− | |backcolour = f5fffa
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− | |linkpage =Immunological testing - WikiBlood
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− | |linktext =IMMUNOLOGICAL TESTING
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− | |sublink1 =Flash Cards - WikiBlood
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− | |subtext1 =WIKIBLOOD FLASHCARDS
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− | |pagetype =Blood
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− | }}
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− | <br>
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| ==<font color="purple">ELISA testing</font>== | | ==<font color="purple">ELISA testing</font>== |
− | {| border="3" cellpadding="8"
| + | <FlashCard questions="3"> |
− | !width="400"|'''Question'''
| + | |q1=What does the acronym ELISA stand for? |
− | !width="400"|'''Answer'''
| + | |a1=Enzyme Linked ImmunoSorbent Assay |
− | !width="150"|'''Article'''
| + | |l1=ELISA testing |
− | |- | + | |q2=What type of ELISA requires all the reagents to be added at once? |
− | |<big>'''What does the acronym ELISA stand for?''' | + | |a2=Homogenous |
− | ||<font color="white"> <big> | + | |l2=ELISA testing |
− | *'''''Enzyme Linked ImmunoSorbent Assay''''' | + | |q3=What are the five basic steps of an ELISA test? |
− | ||[[ELISA testing - WikiBlood|<span title="Answer article">Link to Answer Article</span>]] | + | |a3= |
− | |- | + | *Adsorption of antigen/antibody onto solid phase |
− | |<big>'''What type of ELISA requires all the reagents to be added at once?''' | + | *Addition of chosen sample and reagents |
− | ||<font color="white"> <big> | + | *Incubation and washing |
− | *'''''Homogenous'''''
| + | *Addition of enzyme-labelled antigen/antibody |
− | ||[[ELISA testing - WikiBlood|<span title="Answer article">Link to Answer Article</span>]] | + | *Addition of specific substrate |
| + | |l3=ELISA testing |
| + | </FlashCard> |
| + | |
| + | ==<font color="purple">Immunofluorescence</font>== |
| + | <FlashCard questions="4"> |
| + | |q1=The adsorption of light by a molecule is known as what? |
| + | |a1=Excitation |
| + | |l1=Immunofluorescence |
| + | |q2=What are the fluorescent dyes commonly known as? |
| + | |a2=Fluorochromes |
| + | |l2=Immunofluorescence |
| + | |q3=What are the advantages of indirect staining? |
| + | |a3= |
| + | *Brighter fluorescence |
| + | *One preparation can be used to test many serum samples |
| + | *Relative expressions of different antigens in the same cell can be tested |
| + | *Loss of antibody during conjugation is limited |
| + | |l3=Immunofluorescence |
| + | |q4=What technique is used to provide a quantitative measure of immunofluorescence? |
| + | |a4=Flow cytometry |
| + | |l4=Immunofluorescence |
| + | </FlashCard> |
| + | |
| + | ==<font color="purple">Radioimmunoassay</font>== |
| + | <FlashCard questions="4"> |
| + | |q1=What are radioimmunoassays commonly used to detect? |
| + | |a2= |
| + | *Hormone levels in blood and tissue fluids |
| + | *Serum proteins |
| + | *Drugs |
| + | *Vitamins |
| + | |l1=Radioimmunoassay |
| + | |q2=What is the most common isotope used for radioimmunoassay? |
| + | |a2=Iodine-125 |
| + | |l2=Radioimmunoassay |
| + | |q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? |
| + | |a3=Ensures the number of epitopes present exceeds number of antibody-binding sites |
| + | |l3=Radioimmunoassay |
| + | |q4=What are the disadvantages of the radioimmunoassay? |
| + | |a4= |
| + | *Substances used are radioactive |
| + | *Gamma radiation requires special counting equipment |
| + | *Iodine naturally accumulates in the thyroid gland |
| + | |l4=Radioimmunoassay |
| + | </FlashCard> |
| + | |
| + | ==<font color="purple">Agglutination</font>== |
| + | <FlashCard questions="5"> |
| + | |q1=What are the antibodies that produce agglutination known as? |
| + | |a1=Agglutinins |
| + | |l1=Agglutination |
| + | |q2=What constitutes the end of the agglutination test? |
| + | |a2=The last tube showing distinct agglutination |
| + | |l2=Agglutination |
| + | |q3=What does the titre represent? |
| + | |a3=The number of antibodies per unit volume of serum |
| + | |l3=Agglutination |
| + | |q4=What is the prozone effect? |
| + | |a4=An inhibition of agglutination caused by an excess of antibodies |
| + | |l4=Agglutination |
| + | |q5=What agglutination test is often used to test for rhesus disease? |
| + | |a5=Coombs test |
| + | |l5=Agglutination |
| + | </FlashCard> |
| + | |
| + | |
| + | <br><br> |
| + | {{Jim Bee 2007}} |
| + | [[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]] |